A pond-side test for Guinea worm: Development of a loop-mediated isothermal amplification (LAMP) assay for detection of Dracunculus medinensis.
Africa
Animals
Base Sequence
Cats
Copepoda
/ genetics
DNA Primers
/ chemistry
DNA, Helminth
/ isolation & purification
Disease Vectors
Dogs
Dracunculus Nematode
/ genetics
Humans
Molecular Diagnostic Techniques
/ standards
Nucleic Acid Amplification Techniques
/ standards
Papio
Ponds
/ parasitology
Sensitivity and Specificity
Time Factors
Diagnostics
Dracunculus medinensis
Guinea worm
LAMP
Journal
Experimental parasitology
ISSN: 1090-2449
Titre abrégé: Exp Parasitol
Pays: United States
ID NLM: 0370713
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
03
04
2020
revised:
17
07
2020
accepted:
21
07
2020
pubmed:
7
8
2020
medline:
21
10
2020
entrez:
7
8
2020
Statut:
ppublish
Résumé
Guinea worm Dracunculus medinensis causes debilitating disease in people and is subject to an ongoing global eradication programme. Research and controls are constrained by a lack of diagnostic tools. We developed a specific and sensitive LAMP method for detecting D. medinensis larval DNA in copepod vectors. We were able to detect a single larva in a background of field-collected copepods. This method could form the basis of a "pond-side test" for detecting potential sources of Guinea worm infection in the environment, in copepods, including in the guts of fish as potential transport hosts, enabling research, surveillance and targeting of control measures. The key constraint on the utility of this assay as a field diagnostic, is a lack of knowledge of variation in the temporal and spatial distribution of D. medinensis larvae in copepods in water bodies in the affected areas and how best to sample copepods to obtain a reliable diagnostic sample. These fundamental knowledge gaps could readily be addressed with field collections of samples across areas experiencing a range of worm infection frequencies, coupled with field and laboratory analyses using LAMP and PCR.
Identifiants
pubmed: 32755552
pii: S0014-4894(20)30170-3
doi: 10.1016/j.exppara.2020.107960
pmc: PMC7526612
pii:
doi:
Substances chimiques
DNA Primers
0
DNA, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107960Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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