Efficacy of a Novel Mitochondrial-Derived Peptide in a Porcine Model of Myocardial Ischemia/Reperfusion Injury.
AAR, area-at-risk
Bax, Bcl-2–associated X protein
DAPI, 4′,6-diamidino-2-phenylindole
ELISA, enzyme-linked immunoadsorbent assay
HNG, S14G-humanin analogue
IGFBP3, insulin-like growth factor–binding protein-3
IV, intravenously
LAD, left anterior coronary artery
LV, left ventricular
MDP, mitochondrial-derived peptide
MI, myocardial infarction
MI/R, myocardial ischemia/reperfusion
NIZ, nonischemic zone
RMBF, regional myocardial blood flow
STAT, signal transducer and activator of transcription
TBARS, thiobarbituric acid–reactive substances
TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling
acute myocardial infarction
adjunctive therapy
cTnI, cardiac troponin I
h-FABP, heart fatty acid–binding protein
large animal model
mitochondrial-derived peptide
myocardial ischemia-reperfusion injury
Journal
JACC. Basic to translational science
ISSN: 2452-302X
Titre abrégé: JACC Basic Transl Sci
Pays: United States
ID NLM: 101677259
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
02
03
2020
revised:
23
04
2020
accepted:
25
04
2020
entrez:
8
8
2020
pubmed:
8
8
2020
medline:
8
8
2020
Statut:
epublish
Résumé
With the complexities that surround myocardial ischemia/reperfusion (MI/R) injury, therapies adjunctive to reperfusion that elicit beneficial pleiotropic effects and do not overlap with standard of care are necessary. This study found that the mitochondrial-derived peptide S14G-humanin (HNG) (2 mg/kg), an analogue of humanin, reduced infarct size in a large animal model of MI/R. However, when ischemic time was increased, the infarct-sparing effects were abolished with the same dose of HNG. Thus, although the 60-min MI/R study showed that HNG cardioprotection translates beyond small animal models, further studies are needed to optimize HNG therapy for longer, more patient-relevant periods of cardiac ischemia.
Identifiants
pubmed: 32760857
doi: 10.1016/j.jacbts.2020.04.015
pii: S2452-302X(20)30221-7
pmc: PMC7393416
doi:
Types de publication
Journal Article
Langues
eng
Pagination
699-714Subventions
Organisme : NHLBI NIH HHS
ID : R44 HL139161
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL146098
Pays : United States
Organisme : NHLBI NIH HHS
ID : R56 HL137711
Pays : United States
Organisme : NHLBI NIH HHS
ID : R44 HL139195
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL116571
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK090242
Pays : United States
Informations de copyright
© 2020 The Authors.
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