How to Study the Metabolism of New Psychoactive Substances for the Purpose of Toxicological Screenings-A Follow-Up Study Comparing Pooled Human Liver S9, HepaRG Cells, and Zebrafish Larvae.
LC-HRMS/MS
drugs of abuse
isozyme mapping
metabolism study
toxicological screening
zebrafish
Journal
Frontiers in chemistry
ISSN: 2296-2646
Titre abrégé: Front Chem
Pays: Switzerland
ID NLM: 101627988
Informations de publication
Date de publication:
2020
2020
Historique:
received:
27
02
2020
accepted:
26
05
2020
entrez:
9
8
2020
pubmed:
9
8
2020
medline:
9
8
2020
Statut:
epublish
Résumé
The new psychoactive substances (NPS) market continues to be very dynamic. A large number of compounds belonging to diverse chemical groups continue to emerge. This makes their detection in biological samples challenging for clinical and forensic toxicologists. Knowledge of the metabolic fate of NPS is crucial for developing comprehensive screening procedures. As human studies are not feasible due to ethical concerns, the current study aimed to compare the NPS' metabolic pattern in incubations with pooled human liver S9 fraction (pHLS9), human liver HepaRG cells, and zebrafish larvae. The latter model was recently shown to be a promising preclinical surrogate for human hepatic metabolism of a synthetic cannabinoid. However, studies concerning other NPS classes are still missing and therefore an amphetamine-based
Identifiants
pubmed: 32766204
doi: 10.3389/fchem.2020.00539
pmc: PMC7380166
doi:
Types de publication
Journal Article
Langues
eng
Pagination
539Informations de copyright
Copyright © 2020 Wagmann, Frankenfeld, Park, Herrmann, Fischmann, Westphal, Müller, Flockerzi and Meyer.
Références
Drug Test Anal. 2019 Oct;11(10):1507-1521
pubmed: 31299701
Drug Test Anal. 2014 Jul-Aug;6(7-8):587-97
pubmed: 24995418
J Pharm Biomed Anal. 2019 Sep 10;174:270-276
pubmed: 31181490
Drug Test Anal. 2016 Sep;8(9):891-902
pubmed: 26456305
J Pharmacol Toxicol Methods. 2011 Jan-Feb;63(1):59-68
pubmed: 20460162
J Pharm Biomed Anal. 2017 Sep 5;143:32-42
pubmed: 28601767
Front Chem. 2019 Mar 04;7:109
pubmed: 30886845
Handb Exp Pharmacol. 2018;252:495-541
pubmed: 30105471
Talanta. 2018 Oct 1;188:111-123
pubmed: 30029353
Drug Test Anal. 2019 Feb;11(2):305-317
pubmed: 30160067
Arch Toxicol. 2016 Oct;90(10):2421-44
pubmed: 27665567
Drug Test Anal. 2019 Sep;11(9):1377-1386
pubmed: 31228224
Arch Toxicol. 2013 Aug;87(8):1315-530
pubmed: 23974980
Anal Chim Acta. 2016 Jul 13;927:13-20
pubmed: 27237833
Toxicol Lett. 2016 Sep 6;258:55-70
pubmed: 27320963
Anal Bioanal Chem. 2019 Jul;411(19):4751-4763
pubmed: 30617391
Toxicol Lett. 2018 Mar 15;285:1-8
pubmed: 29277574
Anal Bioanal Chem. 2011 Jul;400(10):3481-9
pubmed: 21533799
Neuropharmacology. 2020 Aug 1;172:107856
pubmed: 31756337
Handb Exp Pharmacol. 2018;252:441-459
pubmed: 29476337
Biochem Pharmacol. 2005 Nov 25;70(11):1673-84
pubmed: 16213467
Arch Toxicol. 2016 Sep;90(9):2161-2172
pubmed: 27369376
Curr Drug Metab. 2019;20(2):91-102
pubmed: 30129408
Drug Metab Dispos. 2011 Aug;39(8):1329-33
pubmed: 21540359
Toxicol Lett. 2015 Jan 5;232(1):159-66
pubmed: 25445008
J Forensic Sci. 2019 Sep;64(5):1451-1461
pubmed: 31260580
Toxicol Lett. 2017 Oct 5;280:142-150
pubmed: 28782580
Handb Exp Pharmacol. 2018;252:413-439
pubmed: 29374833
Chem Biol Interact. 2007 May 20;168(1):66-73
pubmed: 17241619
Drug Test Anal. 2017 Nov;9(11-12):1685-1694
pubmed: 28987069
Toxicol Lett. 2019 May 1;305:73-80
pubmed: 30682400
Drug Metab Dispos. 1998 Jan;26(1):1-4
pubmed: 9443844
Bioanalysis. 2014 Aug;6(15):2083-107
pubmed: 25322784