Theca cell-conditioned medium added to in vitro maturation enhances embryo developmental competence of buffalo (Bubalus bubalis) oocytes after parthenogenic activation.
buffalo
conditioned medium
in vitro maturation
oocytes
theca cells
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
16
03
2020
accepted:
03
08
2020
pubmed:
9
8
2020
medline:
22
6
2021
entrez:
9
8
2020
Statut:
ppublish
Résumé
Theca cells (TCs) play a key role in follicular growth and atresia. TCs synthesize androgens that act as substrate for granulosa cells (GCs) aromatization to estrogens needed for oocyte maturation. However, the effects of TCs in the form of conditioned medium on in vitro maturation (IVM) and developmental competence of buffalo oocytes remain unclear. In the present study, we examined the impacts of TC-conditioned medium (TCCM) on maturation efficiency and embryo development of buffalo oocytes after parthenogenic activation (PA). Our results showed that TCCM that was collected on day 2 and added to IVM medium at a 20% proportional level (2 days & 20%) exerted no significant effect on IVM rate (43.06% vs. 44.71%), but significantly (p < .05) enhanced embryo development (oocyte cleavage, 80.93% vs. 69.66%; blastocyst formation, 39.85% vs. 32.84%) of buffalo oocytes after PA compared with the control group. However, monolayer TC significantly (p < .05) promoted both maturation efficiency (48.84% vs. 44.53%) and embryo development (oocyte cleavage, 80.39% vs. 69.32%; blastocyst formation, 35.38% vs. 29.25%) of buffalo oocytes after PA compared to that in the control group. Furthermore, TCs secreted some testosterone into the conditioned medium, which significantly (p < .05) promoted the expression levels of oestrogen synthesis-related genes (CYP11A1, CYP19A1 and 17β-HSD) in buffalo cumulus-oocyte complexes (COCs). Our study indicated that TCCM (2 days & 20%) did not significantly affect IVM efficiency, but enhanced embryo developmental competence of oocytes after PA principally by stimulating the secretion of testosterone and facilitating estradiol synthesis of buffalo COCs.
Substances chimiques
Culture Media, Conditioned
0
Testosterone
3XMK78S47O
Estradiol
4TI98Z838E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1501-1510Subventions
Organisme : Natural Science Foundation of Guangxi Province
ID : 2018JJA130074
Organisme : The New Century Guangxi Ten, Hundred and Thousand Talent Project
Organisme : Guangxi Innovation-Driven Development Fund Project
ID : AA17204051
Organisme : Nanning Scientific Research and Technological Development Foundation
ID : 20192087
Organisme : Chinese National Natural Science Foundation
ID : 31560633
Organisme : Chinese National Natural Science Foundation
ID : 31760666
Informations de copyright
© 2020 Wiley-VCH GmbH.
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