The functional identification of Dps in oxidative stress resistance and virulence of Riemerella anatipestifer CH-1 using a new unmarked gene deletion strategy.
Animals
Bacterial Proteins
/ genetics
DNA-Binding Proteins
/ genetics
Ducks
/ microbiology
Flavobacteriaceae Infections
/ microbiology
Gene Deletion
Hydrogen Peroxide
/ pharmacology
Iron
/ metabolism
Mutation
Oxidative Stress
Poultry Diseases
/ microbiology
Riemerella
/ drug effects
Virulence
/ genetics
Virulence Factors
/ genetics
DNA binding
Dps
Iron binding
Oxidative stress
Riemerella anatipestifer
Virulence
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
03
03
2020
revised:
05
05
2020
accepted:
19
05
2020
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
8
6
2021
Statut:
ppublish
Résumé
Excessive iron in the bacterial cytoplasm can potentiate the production of harmful reactive oxygen species (ROS). Riemerella anatipestifer (R. anatipestifer, RA), a gram-negative bacterium, encodes an iron uptake system, but its iron detoxification mechanism is unknown. Here, the dps gene of R. anatipestifer CH-1 (RA-CH-1) was deleted using sacB as a counterselection marker. The dps mutant was more sensitive to H
Identifiants
pubmed: 32768200
pii: S0378-1135(20)30255-8
doi: 10.1016/j.vetmic.2020.108730
pii:
doi:
Substances chimiques
Bacterial Proteins
0
DNA-Binding Proteins
0
DPS protein, Bacteria
0
Virulence Factors
0
Hydrogen Peroxide
BBX060AN9V
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108730Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no competing financial interests.