Protective effect of gallic acid and gallic acid-loaded Eudragit-RS 100 nanoparticles on cisplatin-induced mitochondrial dysfunction and inflammation in rat kidney.
Acrylic Resins
/ administration & dosage
Administration, Oral
Animals
Cisplatin
/ administration & dosage
Dose-Response Relationship, Drug
Gallic Acid
/ administration & dosage
Inflammation
/ chemically induced
Injections, Intraperitoneal
Kidney Diseases
/ chemically induced
Male
Mitochondria
/ drug effects
Nanoparticles
/ administration & dosage
Oxidative Stress
/ drug effects
Protective Agents
/ administration & dosage
Rats
Rats, Wistar
Cisplatin
Gallic acid
Inflammation
Mitochondrial dysfunction
Nanoparticle-loaded Eudragit-RS 100
Oxidative stress
Journal
Biochimica et biophysica acta. Molecular basis of disease
ISSN: 1879-260X
Titre abrégé: Biochim Biophys Acta Mol Basis Dis
Pays: Netherlands
ID NLM: 101731730
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
15
05
2020
revised:
02
07
2020
accepted:
03
08
2020
pubmed:
10
8
2020
medline:
20
1
2021
entrez:
10
8
2020
Statut:
ppublish
Résumé
Cisplatin is used as a chemotherapy drug in the treatment of various types of cancer. Mitochondrial dysfunction, oxidative stress and inflammation have been identified as major mechanisms of cisplatin nephrotoxicity. The present study investigated the protective effects of pure gallic acid and nanoparticle gallic acid nanoparticles (nano-gallic acid) on cisplatin induced nephrotoxicity. Nano-gallic acid was prepared by double emulsions-solvent evaporation technique using Eudragit RS 100 polymer and polyvinyl alcohol as carrier. Then, the physicochemical characterization of the nanoparticles was examined. In the present study, renal mitochondria were isolated using different centrifugal methods. Our data indicated that the doses of 50 and 100 mg/kg gallic acid and 10 mg/kg nano-gallic acid significantly decreased mitochondrial reactive oxygen species (ROS) formation, mitochondrial membrane damage (ΔΨm), mitochondrial malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and significantly increased mitochondrial glutathione (GSH), mitochondrial superoxide dismutase (MnSOD), mitochondrial glutathione peroxidase (GPX) and mitochondrial catalase compared to the cisplatin treated group. Histopathological studies also confirmed biochemical tests. Finally, our results confirmed that the pure gallic acid and its nanoparticle improved renal oxidative stress, inflammation and mitochondrial dysfunction in acute nephrotoxicity induced by cisplatin in rat. Nano-gallic acid (10 mg/kg) was selected as the most effective dose. The findings of this study showed the superiority of nano-gallic acid against pure gallic acid. In conclusion, nano-gallic acid-loaded Eudragit-RS 100 as a novel antioxidant can be considered in the treatment of renal complications of cisplatin.
Identifiants
pubmed: 32768679
pii: S0925-4439(20)30259-3
doi: 10.1016/j.bbadis.2020.165911
pii:
doi:
Substances chimiques
Acrylic Resins
0
Protective Agents
0
Eudragit RS
33434-24-1
Gallic Acid
632XD903SP
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
165911Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.