Discovery of the cryptic function of terpene cyclases as aromatic prenyltransferases.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 08 2020
07 08 2020
Historique:
received:
18
03
2020
accepted:
08
07
2020
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
10
9
2020
Statut:
epublish
Résumé
Catalytic versatility is an inherent property of many enzymes. In nature, terpene cyclases comprise the foundation of molecular biodiversity as they generate diverse hydrocarbon scaffolds found in thousands of terpenoid natural products. Here, we report that the catalytic activity of the terpene cyclases AaTPS and FgGS can be switched from cyclase to aromatic prenyltransferase at basic pH to generate prenylindoles. The crystal structures of AaTPS and FgGS provide insights into the catalytic mechanism of this cryptic function. Moreover, aromatic prenyltransferase activity discovered in other terpene cyclases indicates that this cryptic function is broadly conserved among the greater family of terpene cyclases. We suggest that this cryptic function is chemoprotective for the cell by regulating isoprenoid diphosphate concentrations so that they are maintained below toxic thresholds.
Identifiants
pubmed: 32769971
doi: 10.1038/s41467-020-17642-2
pii: 10.1038/s41467-020-17642-2
pmc: PMC7414894
doi:
Substances chimiques
Indoles
0
Ligands
0
Terpenes
0
Dimethylallyltranstransferase
EC 2.5.1.1
Intramolecular Lyases
EC 5.5.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3958Subventions
Organisme : NIH HHS
ID : S10 OD021527
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM125141
Pays : United States
Organisme : NIGMS NIH HHS
ID : P30 GM124165
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103393
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM056838
Pays : United States
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