Morin protects the blood-brain barrier integrity against cerebral ischemia reperfusion through anti-inflammatory actions in rats.
Animals
Anti-Inflammatory Agents
Apoptosis
/ drug effects
Blood-Brain Barrier
/ drug effects
Brain Ischemia
/ drug therapy
Disease Models, Animal
Flavonoids
/ administration & dosage
Inflammation
Injections, Intraperitoneal
Lipid Peroxidation
/ drug effects
Male
NF-kappa B
/ metabolism
Neutrophil Infiltration
/ drug effects
Oxidative Stress
/ drug effects
Rats, Wistar
Reactive Oxygen Species
/ metabolism
Reperfusion Injury
/ prevention & control
Toll-Like Receptor 4
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 08 2020
07 08 2020
Historique:
received:
24
10
2019
accepted:
20
07
2020
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
10
2
2021
Statut:
epublish
Résumé
This study aimed to investigate the effects of morin on cerebral damage and blood-brain barrier (BBB) integrity in a middle cerebral artery occlusion (MCAO) and reperfusion model. Wistar rats were exposed to MCAO for 2 h, followed by reperfusion. Thirty mg/kg of morin was administered via intraperitoneal injection at the different time points: before ischemia, during ischemia, and at reperfusion. The rats were divided into five groups, including sham, vehicle, and three groups of morin. Twenty-four hours after reperfusion, the rats were tested for neurological deficits, and the brains were harvested to assess brain damage. In addition, brains were harvested 72 h to determine BBB disruption. We found that morin significantly reduced reactive oxygen species production and lipid peroxidation. It also decreased inflammation via reducing the expression of Toll-like receptor 4, nuclear factor kappa-beta. Morin ameliorated cerebral damage and reduced apoptosis through decreasing the cerebral infarct size, including apoptotic cell death. Moreover, morin decreased the BBB damage via reducing Evans blue extravasation, neutrophil infiltration, and increasing tight junction protein expression. Therefore, morin protected against cerebral and BBB damage by attenuating oxidative stress, inflammation, and apoptosis in MCAO and reperfusion models.
Identifiants
pubmed: 32770144
doi: 10.1038/s41598-020-70214-8
pii: 10.1038/s41598-020-70214-8
pmc: PMC7414849
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Flavonoids
0
NF-kappa B
0
Reactive Oxygen Species
0
Toll-Like Receptor 4
0
morin
8NFQ3F76WR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13379Références
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