Aqueous two-phase partitioning and characterization of xylanase produced by Streptomyces geysiriensis from low cost lignocellulosic substrates.
Aqueous two-phase systems
Lignocellulosic substrates
Solid-state fermentation
Streptomyces geysiriensis
Xylanase
Journal
Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
27
04
2020
revised:
06
07
2020
accepted:
12
07
2020
pubmed:
11
8
2020
medline:
19
12
2020
entrez:
11
8
2020
Statut:
ppublish
Résumé
Microbial production of xylanase is gaining the commercial importance, due to its wide range of applications from paper and pulp to food and feed industries. Streptomyces geysiriensis was used for the production of extracellular xylanase from lignocellulosic substrates such as rice bran and saw dust, under solid-state fermentation. The influence of pH, temperature and incubation period for the maximum production of xylanase was investigated with 1:2 (w/v) of substrate to moisture ratio at 100 rpm shaking conditions. The maximum production was recorded after 5 days of fermentation with pH 8.0 at 40 °C. The scale-up was done based on the results of optimized parameters using 3 L Applikon autoclavable bioreactor with maximum yield of 186 U/ml after 4 days of fermentation. Extracellular xylanase was separated by partitioning in aqueous two-phase system consisting of 20% polyethylene glycol 6000 and 12% K
Identifiants
pubmed: 32773265
pii: S1389-1723(20)30288-7
doi: 10.1016/j.jbiosc.2020.07.008
pii:
doi:
Substances chimiques
Xylans
0
Water
059QF0KO0R
lignocellulose
11132-73-3
Lignin
9005-53-2
Endo-1,4-beta Xylanases
EC 3.2.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
571-576Informations de copyright
Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.