Expression and purification of the extracellular domain of wild-type humanRET and the dimeric oncogenic mutant C634R.
Animals
Carcinogenesis
/ genetics
Cell Line
Cysteine
/ genetics
Disulfides
/ metabolism
HEK293 Cells
Hirschsprung Disease
/ genetics
Humans
Mammals
/ genetics
Multiple Endocrine Neoplasia Type 2a
/ genetics
Mutation
/ genetics
Parkinson Disease
/ genetics
Protein Domains
/ genetics
Proto-Oncogene Proteins c-ret
/ genetics
Recombinant Proteins
/ genetics
Signal Transduction
/ genetics
Cysteine-rich domain
Receptor tyrosine kinase
Recombinant protein expression
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Dec 2020
01 Dec 2020
Historique:
received:
24
05
2020
revised:
15
07
2020
accepted:
24
07
2020
pubmed:
11
8
2020
medline:
10
4
2021
entrez:
11
8
2020
Statut:
ppublish
Résumé
The receptor tyrosine kinase RET is essential in a variety of cellular processes. RET gain-of-function is strongly associated with several cancers, notably multiple endocrine neoplasia type 2A (MEN 2A), while RET loss-of-function causes Hirschsprung's disease and Parkinson's disease. To investigate the activation mechanism of RET as well as to enable drug development, over-expressed recombinant protein is needed for in vitro functional and structural studies. By comparing insect and mammalian cells expression of the RET extracellular domain (RET
Identifiants
pubmed: 32777409
pii: S0141-8130(20)33983-0
doi: 10.1016/j.ijbiomac.2020.07.290
pii:
doi:
Substances chimiques
Disulfides
0
Recombinant Proteins
0
Proto-Oncogene Proteins c-ret
EC 2.7.10.1
Cysteine
K848JZ4886
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1621-1630Informations de copyright
Copyright © 2020. Published by Elsevier B.V.