Gastric healing effect of p-coumaric acid isolated from Baccharis dracunculifolia DC on animal model.
Acetic Acid
Animals
Anti-Ulcer Agents
/ pharmacology
Baccharis
/ chemistry
Catalase
/ metabolism
Cell Line
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Coumaric Acids
/ isolation & purification
Disease Models, Animal
Female
Gastric Mucosa
/ drug effects
Glutathione
/ metabolism
Humans
Mice
Peroxidase
/ metabolism
Phytotherapy
Rats
Stomach Ulcer
/ chemically induced
Superoxide Dismutase
/ metabolism
Gastric healing
Gastric ulcer
Oxidative stress
Phenolic compound
Journal
Naunyn-Schmiedeberg's archives of pharmacology
ISSN: 1432-1912
Titre abrégé: Naunyn Schmiedebergs Arch Pharmacol
Pays: Germany
ID NLM: 0326264
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
28
03
2020
accepted:
18
06
2020
pubmed:
12
8
2020
medline:
21
10
2021
entrez:
12
8
2020
Statut:
ppublish
Résumé
The p-coumaric acid is a phenolic compound present in large quantities in the extract of Baccharis dracunculifolia DC, a Brazilian medicinal plant used to treat gastric ulcer. Given the necessity for finding new chemical components capable of accelerating gastric healing, in this study, the effects of the p-coumaric acid were evaluated in the acetic acid-induced ulcer model in rats, where histological, inflammatory, and oxidative parameters were analyzed. The healing property was also evaluated in the scratch assay on fibroblast cells (L929) and the cytotoxicity of p-coumaric acid was assessed in both L929 and human gastric adenocarcinoma (AGS) cells by MTT assay. The treatment with p-coumaric acid (10 mg/kg, p.o.) for 7 days, twice a day, decreased by 44.6% the acetic acid-induced gastric ulcer compared with the vehicle-treated group. The vehicle control-treated group showed a larger extension of the ulcer base and an extensive damage into the mucosa and submucosa layers, which were mitigated by the treatment with p-coumaric acid. This beneficial effect was also associated with increased levels of mucin and reduced glutathione, decreased amount of lipid hydroperoxides, and increased superoxide dismutase and catalase activities without interfering with the activity of myeloperoxidase in the gastric tissue. The compound promoted the restructuring of the cell monolayer in the scratch test and did not show toxicity in the L929 cell line, while reduced the viability of the AGS, a lineage of human gastric adenocarcinoma. Thus, p-coumaric acid may be considered a natural source for the treatment of gastric ulcers, by reinforcing protective factors of gastric mucosa and by accelerating gastric healing.
Identifiants
pubmed: 32780226
doi: 10.1007/s00210-020-01928-9
pii: 10.1007/s00210-020-01928-9
doi:
Substances chimiques
Anti-Ulcer Agents
0
Coumaric Acids
0
Catalase
EC 1.11.1.6
Peroxidase
EC 1.11.1.7
Superoxide Dismutase
EC 1.15.1.1
Glutathione
GAN16C9B8O
p-coumaric acid
IBS9D1EU3J
Acetic Acid
Q40Q9N063P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
49-57Références
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