Photocycle of Cyanobacteriochrome TePixJ.

Bacterial Proteins / chemistry Cyanobacteria / metabolism Kinetics Light Models, Molecular Protein Conformation

Journal

Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623

Informations de publication

Date de publication:
18 08 2020
Historique:
pubmed: 14 8 2020
medline: 13 3 2021
entrez: 14 8 2020
Statut: ppublish

Résumé

Due to the recent advances in X-ray free electron laser techniques, bilin-containing cyanobacteriochrome photoreceptors have become prime targets for the ever-expanding field of time-resolved structural biology. However, to facilitate these challenging studies, it is essential that the time scales of any structural changes during the photocycles of cyanobacteriochromes be established. Here, we have used visible and infrared transient absorption spectroscopy to probe the photocycle of a model cyanobacteriochrome system, TePixJ. The kinetics span multiple orders of magnitude from picoseconds to seconds. Localized changes in the bilin binding pocket occur in picoseconds to nanoseconds, followed by more large-scale changes in protein structure, including formation and breakage of a second thioether linkage, in microseconds to milliseconds. The characterization of the entire photocycle will provide a vital frame of reference for future time-resolved structural studies of this model photoreceptor.

Identifiants

DOI: 10.1021/acs.biochem.0c00382 PMID: 32786403 PMC: PMC7450660
pubmed: 32786403
doi: 10.1021/acs.biochem.0c00382
pmc: PMC7450660
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2909-2915

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011658/1
Pays : United Kingdom

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Auteurs

Samantha J O Hardman (SJO)

Manchester Institute of Biotechnology and Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Derren J Heyes (DJ)

Manchester Institute of Biotechnology and Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.

Igor V Sazanovich (IV)

Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Harwell Oxford, Didcot OX11 0QX, United Kingdom.

Nigel S Scrutton (NS)

Manchester Institute of Biotechnology and Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, The University of Manchester, 131 Princess Street, Manchester M1 7DN, U.K.
ORCID: 0000-0002-4182-3500

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Photocycle of Cyanobacteriochrome TePixJ.
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries à Gram négatif photosynthétiques › Cyanobactéries › Photocycle of Cyanobacteriochrome TePixJ.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Photocycle of Cyanobacteriochrome TePixJ.
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Photocycle of Cyanobacteriochrome TePixJ.
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Photocycle of Cyanobacteriochrome TePixJ.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Photocycle of Cyanobacteriochrome TePixJ.