MiR-21-5p but not miR-1-3p expression is modulated by preconditioning in a rat model of myocardial infarction.
Animals
Apoptosis Regulatory Proteins
/ genetics
Disease Models, Animal
Heme Oxygenase-1
/ genetics
Hypoxia-Inducible Factor 1, alpha Subunit
/ genetics
Ischemic Preconditioning
Isoflurane
/ analogs & derivatives
Male
MicroRNAs
/ genetics
Myocardial Infarction
/ genetics
Myocardial Reperfusion Injury
/ genetics
Rats
Rats, Wistar
Real-Time Polymerase Chain Reaction
Signal Transduction
/ drug effects
Vascular Endothelial Growth Factor A
/ genetics
HIF1α
Isoflurane
Preconditioning
miR-1-3p
miR-21-5p
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
19
05
2020
accepted:
02
08
2020
pubmed:
14
8
2020
medline:
26
5
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
Isoflurane (Iso) preconditioning (PC) is known to be cardioprotective against ischemia/reperfusion (I/R) injury. It was previously shown that microRNA-21-5p (miR-21-5p) is regulated by Iso-PC. It is unclear, if expression of cardiac enriched miR-1-3p is also affected by Iso-PC, and associated with activation of HIF1α (hypoxia-inducible factor 1-alpha). Male Wistar rats (n = 6-8) were randomly assigned to treatment with or without 1 MAC Iso for 30 min, followed by 25 min of regional myocardial ischemia, with 120 min reperfusion. At the end of reperfusion, myocardial expression of miR-1-3p, miR-21-5p and mRNAs of two HIF-1α-dependent genes, VEGF (vascular endothelial growth factor) and HO-1 (heme oxygenase-1), were determined by quantitative PCR. Protein expression of a miR-21 target gene, PDCD4 (programmed cell death protein 4), was assessed by western blot analysis. Infarct sizes were analyzed with triphenyltetrazoliumchloride staining. MiR-21-5p expression was increased by Iso, whereas expression of miR-1-3p was not altered. The expression of VEGF but not HO-1 was induced by Iso. Iso-PC reduced infarct sizes compared to untreated controls. No regulation of miRNA and mRNA expression was detected after I/R. PDCD4 protein expression was not affected after Iso exposure. Expression of miR-21-5p, in contrast to miR-1-3p, is altered during this early time point of Iso-PC. HIF1α signaling seems to be involved in miR-21-5p regulation.
Identifiants
pubmed: 32789575
doi: 10.1007/s11033-020-05721-y
pii: 10.1007/s11033-020-05721-y
pmc: PMC7561583
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
MIRN1 microRNA, rat
0
MicroRNAs
0
Pdcd4 protein, rat
0
Vascular Endothelial Growth Factor A
0
mirn21 microRNA, rat
0
Isoflurane
CYS9AKD70P
Heme Oxygenase-1
EC 1.14.14.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6669-6677Références
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