Rad54 and Rdh54 occupy spatially and functionally distinct sites within the Rad51-ssDNA presynaptic complex.
Binding Sites
Catalytic Domain
/ genetics
Cell Cycle Proteins
/ genetics
Chromosome Pairing
DNA Helicases
/ genetics
DNA Repair
/ genetics
DNA Repair Enzymes
/ genetics
DNA Topoisomerases
/ genetics
DNA, Single-Stranded
/ metabolism
DNA-Binding Proteins
/ metabolism
Mutation
Protein Binding
Protein Domains
Rad51 Recombinase
/ genetics
Recombinant Proteins
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ genetics
DNA repair
Rad51
Rad54
Rdh54
homologous recombination
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 10 2020
15 10 2020
Historique:
received:
21
05
2020
revised:
13
07
2020
accepted:
29
07
2020
pubmed:
14
8
2020
medline:
15
4
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
Rad54 and Rdh54 are closely related ATP-dependent motor proteins that participate in homologous recombination (HR). During HR, these enzymes functionally interact with the Rad51 presynaptic complex (PSC). Despite their importance, we know little about how they are organized within the PSC, or how their organization affects PSC function. Here, we use single-molecule optical microscopy and genetic analysis of chimeric protein constructs to evaluate the binding distributions of Rad54 and Rdh54 within the PSC. We find that Rad54 and Rdh54 have distinct binding sites within the PSC, which allow these proteins to act cooperatively as DNA sequences are aligned during homology search. Our data also reveal that Rad54 must bind to a specific location within the PSC, whereas Rdh54 retains its function in the repair of MMS-induced DNA damage even when recruited to the incorrect location. These findings support a model in which the relative binding sites of Rad54 and Rdh54 help to define their functions during mitotic HR.
Identifiants
pubmed: 32790929
doi: 10.15252/embj.2020105705
pmc: PMC7560196
doi:
Substances chimiques
Cell Cycle Proteins
0
DNA, Single-Stranded
0
DNA-Binding Proteins
0
Hed1 protein, S cerevisiae
0
Recombinant Proteins
0
Saccharomyces cerevisiae Proteins
0
RAD51 protein, S cerevisiae
EC 2.7.7.-
Rad51 Recombinase
EC 2.7.7.-
RAD54 protein, S cerevisiae
EC 3.6.1.-
DNA Helicases
EC 3.6.4.-
DNA Topoisomerases
EC 5.99.1.-
RDH54 protein, S cerevisiae
EC 5.99.1.-
DNA Repair Enzymes
EC 6.5.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e105705Subventions
Organisme : Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation)
ID : DRG 2310-17
Organisme : NCI NIH HHS
ID : P01 CA092584
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118026
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA054174
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES007061
Pays : United States
Organisme : NSF|BIO|Division of Molecular and Cellular Biosciences (MCB)
ID : MCB1154511
Organisme : NCI NIH HHS
ID : R35 CA241801
Pays : United States
Informations de copyright
© 2020 The Authors.
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