Purification, isolation, and structure characterization of water soluble and insoluble polysaccharides from Maitake fruiting body.

Chromatography, Gel / methods Chromatography, Ion Exchange / methods Fruiting Bodies, Fungal / chemistry Fungal Polysaccharides / chemistry Grifola / chemistry Magnetic Resonance Spectroscopy / methods Polysaccharides / chemistry Water / analysis
Polysaccharides Purification Structure characterization

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
01 Dec 2020
Historique:
received: 12 06 2020
revised: 31 07 2020
accepted: 04 08 2020
pubmed: 14 8 2020
medline: 10 4 2021
entrez: 14 8 2020
Statut: ppublish

Résumé

The crude polysaccharides (GFP) were isolated from the Maitake fruiting body (Grifola frondosa) and purified by DEAE Cellulose-52 ionic-exchange chromatography and Sephadex G-25 gel filtration chromatography in that order. Five main fractions, GFP-1 to GFP-5 were obtained through the isolation and purification steps. Free sugars were isolated by G-25 gel filtration chromatography and identified glucose and (α,α)-trehalose by nuclear magnetic resonance (NMR). GC-MS and methylation analysis that linkages were mainly β-1,3 and β-1,6, β-1,4 and β-1,2 bonds in WIP. Seven main oligomer products were detected and their structures characterized by mass spectrum. Experimental results shown the similarity in structure between water soluble polysaccharides (WSP) and water insoluble polysaccharides (WIP), thus WSP can be the product of cell wall by breakdown.

Identifiants

DOI: 10.1016/j.ijbiomac.2020.08.037 PMID: 32791276
pubmed: 32791276
pii: S0141-8130(20)34124-6
doi: 10.1016/j.ijbiomac.2020.08.037
pii:
doi:

Substances chimiques

Fungal Polysaccharides 0
Polysaccharides 0
Water 059QF0KO0R

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1879-1888

Informations de copyright

Copyright © 2020. Published by Elsevier B.V.

Auteurs

Mst Nushrat Yiasmin (MN)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.

Md Serajul Islam (MS)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.

He He (H)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.

Yaxian Liu (Y)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.

Mingming Wang (M)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.

Ruijin Yang (R)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China.

Xiao Hua (X)

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: huaxiao@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie sur gel › Purification, isolation, and structure...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'échange d'ions › Purification, isolation, and structure...
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questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes fongiques › Polysaccharides fongiques › Purification, isolation, and structure...
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questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Purification, isolation, and structure...
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