Progress in molecular detection with high-speed nucleic acids thermocyclers.

Heating Nucleic Acids / analysis Polymerase Chain Reaction
High-speed Nucleic acids detection Polymerase chain reaction Thermal cycling

Journal

Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336

Informations de publication

Date de publication:
25 Oct 2020
Historique:
received: 28 04 2020
revised: 17 07 2020
accepted: 20 07 2020
pubmed: 14 8 2020
medline: 18 5 2021
entrez: 14 8 2020
Statut: ppublish

Résumé

Polymerase chain reaction (PCR) technology is regarded as the gold standard in the area of nucleic acids detection due to its high sensitivity and specificity. The utility of PCR, however, is somehow hampered by the fact that it is time-consuming (generally more than 60 min). Faster heating cycles are therefore needed to shorten the reaction times and improve the detection efficiency. In this review, the kinetics of PCR are first analyzed from three reaction stages, namely template denaturation, primmer annealing and polymerase extension. The PCR can be conducted quickly without sacrificing the amplification efficiency and sensitivity by optimizing the instruments and biochemical reaction. Then, the characteristics and progresses of high-speed nucleic acids thermocyclers are fully analyzed and discussed. And the thermocyclers are mainly divided into contact thermocyclers and non-contact thermocyclers. Finally, different aspects of high-speed PCR including thermocycling, biochemistry, signal identification and integrated device are prospected. With the development of technology, the high-speed thermocyclers would become more and more mature and it would play a more important role in the molecular diagnostics, especially for time-sensitive detection areas.

Identifiants

DOI: 10.1016/j.jpba.2020.113489 PMID: 32791435
pubmed: 32791435
pii: S0731-7085(20)31375-3
doi: 10.1016/j.jpba.2020.113489
pii:
doi:

Substances chimiques

Nucleic Acids 0

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

113489

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no conflict of interest.

Auteurs

Hui Wu (H)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Shiyue Zhang (S)

Biochemistry Program, Binghamton University, State University of New York, 4400 Vestal Parkway East, Binghamton, NY 13902, United States.

Yanju Chen (Y)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Cheng Qian (C)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Yang Liu (Y)

Key Laboratory of Microbiol Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China.

Hong Shen (H)

NMPA Key Laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Biological Inspection Department, Zhejiang Institute for Food and Drug Control, Hangzhou, 310052, China.

Zhijian Wang (Z)

NMPA Key Laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Biological Inspection Department, Zhejiang Institute for Food and Drug Control, Hangzhou, 310052, China.

Jianfeng Ping (J)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Jian Wu (J)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture, Hangzhou, 310058, China. Electronic address: wujian69@zju.edu.cn.

Yanjun Zhang (Y)

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China. Electronic address: yjzhang@cdc.zj.cn.

Huan Chen (H)

Key Laboratory of Microbiol Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, 310012, China. Electronic address: chenhuan7809@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Environnement contrôlé › Chauffage › Progress in molecular detection with...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › Progress in molecular detection with...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Progress in molecular detection with...