A cyclic tetrasaccharide, cycloisomaltotetraose, was enzymatically produced from dextran and its crystal structure was determined.
Cycloisomaltooligosaccharide
Cycloisomaltotetraose
Transglycosylation
X-ray crystallographic structure
α-1,6 linkage
Journal
Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
18
05
2020
revised:
30
06
2020
accepted:
14
07
2020
pubmed:
17
8
2020
medline:
22
6
2021
entrez:
16
8
2020
Statut:
ppublish
Résumé
Two bacterial strains isolated from soil, namely Agreia sp. D1110 and Microbacterium trichothecenolyticum D2006, were found to produce a novel oligosaccharide. The oligosaccharide was enzymatically produced from dextran using the culture supernatant of Agreia sp. D1110 or M. trichothecenolyticum D2006. LC-MS and NMR analysis identified the novel oligosaccharide as cyclo-{→6)-α-d-Glcp-(1→6)-α-d-Glcp-(1→6)-α-d-Glcp-(1→6)-α-d-Glcp-(1→}, which was named cycloisomaltotetraose, and abbreviated as CI4. CI4 was subsequently crystalized and its X-ray crystallographic structure was determined. CI4 crystals were shown to be pentahydrate, with the CI4 molecules in the crystal structure displaying a unique 3D structure, in which two glucosyl residues in the molecule were facing each other. This unique 3D structure was quite different from the 3D structure of known cyclic tetrasaccharides. This is the first report of CI4 molecules and their unique crystal structure.
Identifiants
pubmed: 32795710
pii: S0008-6215(20)30308-6
doi: 10.1016/j.carres.2020.108104
pii:
doi:
Substances chimiques
Dextrans
0
Oligosaccharides
0
isomaltotetraose
35997-20-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108104Informations de copyright
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