Multiple-Labeled Antibodies Behave Like Single Emitters in Photoswitching Buffer.

Antibodies Carbocyanines Fluorescent Dyes Microscopy, Fluorescence Photons
dSTORM energy hopping photon antibunching single-molecule photophysics super-resolution microscopy

Journal

ACS nano
ISSN: 1936-086X
Titre abrégé: ACS Nano
Pays: United States
ID NLM: 101313589

Informations de publication

Date de publication:
27 10 2020
Historique:
pubmed: 18 8 2020
medline: 15 5 2021
entrez: 18 8 2020
Statut: ppublish

Résumé

The degree of labeling (DOL) of antibodies has so far been optimized for high brightness and specific and efficient binding. The influence of the DOL on the blinking performance of antibodies used in

Identifiants

DOI: 10.1021/acsnano.0c06099 PMID: 32804475
pubmed: 32804475
doi: 10.1021/acsnano.0c06099
doi:

Substances chimiques

Antibodies 0
Carbocyanines 0
Fluorescent Dyes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

12629-12641

Auteurs

Dominic A Helmerich (DA)

Department of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.

Gerti Beliu (G)

Department of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.

Markus Sauer (M)

Department of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.
ORCID: 0000-0002-1692-3219

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Multiple-Labeled Antibodies Behave Like Single...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcynes › Carbocyanines › Multiple-Labeled Antibodies Behave Like Single...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Multiple-Labeled Antibodies Behave Like Single...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Multiple-Labeled Antibodies Behave Like Single...
questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Photons › Multiple-Labeled Antibodies Behave Like Single...