The Effect of Leucine-Enriched Essential Amino Acid Supplementation on Anabolic and Catabolic Signaling in Human Skeletal Muscle after Acute Resistance Exercise: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Comparison Trial.
Amino Acids, Essential
/ pharmacology
Cytokines
/ metabolism
Dietary Supplements
Double-Blind Method
Exercise
/ physiology
Healthy Volunteers
Humans
Interleukin-1beta
/ metabolism
Interleukin-6
/ metabolism
Leucine
/ pharmacology
Male
Mechanistic Target of Rapamycin Complex 1
/ metabolism
Muscle Proteins
/ metabolism
Muscle, Skeletal
/ metabolism
Phosphorylation
/ drug effects
RNA, Messenger
/ metabolism
Resistance Training
SKP Cullin F-Box Protein Ligases
/ metabolism
Signal Transduction
/ drug effects
Tripartite Motif Proteins
/ metabolism
Ubiquitin-Protein Ligases
/ metabolism
Young Adult
LEAAs
inflammation
mTORC1
resistance exercise
ubiquitin
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
12 Aug 2020
12 Aug 2020
Historique:
received:
24
06
2020
revised:
28
07
2020
accepted:
03
08
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
17
3
2021
Statut:
epublish
Résumé
Resistance exercise transiently activates anabolic and catabolic systems in skeletal muscle. Leucine-enriched essential amino acids (LEAAs) are reported to stimulate the muscle anabolic response at a lower dose than whey protein. However, little is known regarding the effect of LEAA supplementation on the resistance exercise-induced responses of the anabolic and catabolic systems. Here, we conducted a randomized, double-blind, placebo-controlled, parallel-group comparison trial to investigate the effect of LEAA supplementation on mechanistic target of rapamycin complex 1 (mTORC1), the ubiquitin-proteasome system and inflammatory cytokines after a single bout of resistance exercise in young men. A total of 20 healthy young male subjects were supplemented with either 5 g of LEAA or placebo, and then they performed 10 reps in three sets of leg extensions and leg curls (70% one-repetition maximum). LEAA supplementation augmented the phosphorylation of mTOR
Identifiants
pubmed: 32806711
pii: nu12082421
doi: 10.3390/nu12082421
pmc: PMC7468954
pii:
doi:
Substances chimiques
Amino Acids, Essential
0
Cytokines
0
IL1B protein, human
0
IL6 protein, human
0
Interleukin-1beta
0
Interleukin-6
0
Muscle Proteins
0
RNA, Messenger
0
Tripartite Motif Proteins
0
FBXO32 protein, human
EC 2.3.2.27
SKP Cullin F-Box Protein Ligases
EC 2.3.2.27
TRIM63 protein, human
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Mechanistic Target of Rapamycin Complex 1
EC 2.7.11.1
Leucine
GMW67QNF9C
Types de publication
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ajinomoto Co., Inc.
ID : Not applicable
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