Impact of a dry inoculum deposition on the efficacy of copper-based antimicrobial surfaces.


Journal

The Journal of hospital infection
ISSN: 1532-2939
Titre abrégé: J Hosp Infect
Pays: England
ID NLM: 8007166

Informations de publication

Date de publication:
Nov 2020
Historique:
received: 21 04 2020
accepted: 11 08 2020
pubmed: 19 8 2020
medline: 2 7 2021
entrez: 19 8 2020
Statut: ppublish

Résumé

The introduction of antimicrobial surfaces into healthcare environments is believed to impact positively on the rate of healthcare-associated infections by significantly decreasing pathogen presence on surfaces. To report on a novel efficacy test that uses a dry bacterial inoculum to measure the microbicidal efficacy of antimicrobial surfaces. An aerosolized dry inoculum of Staphylococcus aureus or Acinetobacter baumannii was deposited on copper alloy surfaces or a hospital-grade stainless-steel surface. Surviving bacteria were enumerated following incubation of the inoculated surfaces at an environmentally relevant temperature and relative humidity. Damage caused to bacteria by the aerosolization process and by the different surfaces was investigated. Dry inoculum testing showed a <2-log Our test provided a realistic deposition of a bacterial inoculum to a surface and, as such, a realistic protocol to assess the efficacy of dry antimicrobial environmental surfaces in vitro.

Sections du résumé

BACKGROUND BACKGROUND
The introduction of antimicrobial surfaces into healthcare environments is believed to impact positively on the rate of healthcare-associated infections by significantly decreasing pathogen presence on surfaces.
AIM OBJECTIVE
To report on a novel efficacy test that uses a dry bacterial inoculum to measure the microbicidal efficacy of antimicrobial surfaces.
METHODS METHODS
An aerosolized dry inoculum of Staphylococcus aureus or Acinetobacter baumannii was deposited on copper alloy surfaces or a hospital-grade stainless-steel surface. Surviving bacteria were enumerated following incubation of the inoculated surfaces at an environmentally relevant temperature and relative humidity. Damage caused to bacteria by the aerosolization process and by the different surfaces was investigated.
FINDINGS RESULTS
Dry inoculum testing showed a <2-log
CONCLUSIONS CONCLUSIONS
Our test provided a realistic deposition of a bacterial inoculum to a surface and, as such, a realistic protocol to assess the efficacy of dry antimicrobial environmental surfaces in vitro.

Identifiants

pubmed: 32810570
pii: S0195-6701(20)30391-1
doi: 10.1016/j.jhin.2020.08.013
pii:
doi:

Substances chimiques

Aerosols 0
Alloys 0
Anti-Bacterial Agents 0
Anti-Infective Agents 0
Coated Materials, Biocompatible 0
Copper 789U1901C5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

465-472

Informations de copyright

Copyright © 2020. Published by Elsevier Ltd.

Auteurs

M McDonald (M)

Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, Wales, UK.

R Wesgate (R)

Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, Wales, UK.

M Rubiano (M)

Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, Wales, UK.

J Holah (J)

Holchem Laboratories Ltd, Bury, UK.

S P Denyer (SP)

University of Brighton, Cockcroft Building, Brighton, UK.

C Jermann (C)

GNT UK Ltd, Derby, UK.

J-Y Maillard (JY)

Cardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, Wales, UK. Electronic address: maillardj@cardiff.ac.uk.

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