Morphological and molecular identification of Hysterothylacium larvae (Nematoda: Raphidascarididae) in marine fish from Tunisian Mediterranean coasts.
Hysterothylacium type V
Mediterranean Sea
Sequencing
mtDNA cox2 gene
rDNA ITS regions
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
14
03
2019
accepted:
09
08
2020
pubmed:
20
8
2020
medline:
21
10
2020
entrez:
20
8
2020
Statut:
ppublish
Résumé
The taxonomy of Hysterothylacium genus in Mediterranean waters remains incomplete and unresolved. The aim of the current study was to investigate the morphological and molecular identification of selected species of Hysterothylacium larvae in marine fish from the Tunisian Mediterranean coasts. A total of 192 marine fish samples were examined. In total, thirty-seven third-stage larvae of Hysterothylacium were morphologically identified as Hysterothylacium type V. In the present study, representatives of this type from the Mediterranean Sea were genetically characterized for the first time by sequencing the rDNA ITS (ITS1-5.8S-ITS2) regions and mtDNA cox2 gene. This study represents the first report of Hysterothylacium type V from the Mediterranean Sea. We also report Mullus barbatus, M. surmuletus, and Pagellus erythrinus as new hosts for this larval type. Based upon molecular and phylogenetic analyses considering the rDNA ITS regions, the Hysterothylacium type V described here was classified as a new genotype, named Genotype B. The valid genetic data of the described Hysterothylacium type V in the present study can be used to establish the phylogenetic relationships among Hysterothylacium species from the Mediterranean Sea and worldwide for future research.
Identifiants
pubmed: 32812144
doi: 10.1007/s00436-020-06848-4
pii: 10.1007/s00436-020-06848-4
doi:
Substances chimiques
DNA, Mitochondrial
0
DNA, Ribosomal Spacer
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3285-3296Références
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