Blood flow restriction in human skeletal muscle during rest periods after high-load resistance training down-regulates miR-206 and induces Pax7.
Blood flow restriction
High-intensity resistance training
Satellite cells
microRNA
Journal
Journal of sport and health science
ISSN: 2213-2961
Titre abrégé: J Sport Health Sci
Pays: China
ID NLM: 101606001
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
04
02
2019
revised:
24
05
2019
accepted:
26
06
2019
pubmed:
20
8
2020
medline:
22
9
2021
entrez:
20
8
2020
Statut:
ppublish
Résumé
Blood flow restriction (BFR) with low-intensity resistance training has been shown to result in hypertrophy of skeletal muscle. In this study, we tested the hypothesis that BFR during the rest periods between acute, high-intensity resistance exercise sessions (70% of 1 repetition maximum, 7 sets with 10 repetitions) enhances the effects of the resistance training. A total of 7 healthy young men performed squats, and between sets BFR was carried out on one leg while the other leg served as a control. Because BFR was applied during rest periods, even severe occlusion pressure (approximately 230 mmHg), which almost completely blocked blood flow, was well-tolerated by the participants. Five muscle-specific microRNAs were measured from the biopsy samples, which were taken 2 h after the acute training. Doppler data showed that the pattern of blood flow recovery changed significantly between the first and last BFR. microRNA-206 levels significantly decreased in the BFR leg compared to the control. The mRNA levels of RAC-β serine/threonine-protein kinase v22, nuclear respiratory factor 1, vascular endothelial growth factor, lupus Ku autoantigen protein p70 genes (p < 0.05), and paired box 7 (p < 0.01) increased in the BFR leg. The protein levels of paired box 7, nuclear respiratory factor 1, and peroxisome proliferator-activated receptor γ coactivator 1α did not differ between the BFR leg and the control leg. BFR, during the rest periods of high-load resistance training, could lead to mRNA elevation of those proteins that regulate angiogenesis, mitochondrial biogenesis, and muscle hypertrophy and repair. However, BFR also can cause DNA damage, judging from the increase in mRNA levels of lupus Ku autoantigen protein p70.
Identifiants
pubmed: 32813644
pii: S2095-2546(19)30105-X
doi: 10.1016/j.jshs.2019.08.004
pmc: PMC8343007
pii:
doi:
Substances chimiques
MIRN206 microRNA, human
0
MicroRNAs
0
PAX7 Transcription Factor
0
PAX7 protein, human
0
Ku Autoantigen
EC 4.2.99.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
470-477Informations de copyright
Copyright © 2021. Production and hosting by Elsevier B.V.
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