A CRISPRi-dCas9 System for Archaea and Its Use To Examine Gene Function during Nitrogen Fixation by Methanosarcina acetivorans.

Archaeal Proteins / genetics CRISPR-Cas Systems Gene Expression Genes, Archaeal / genetics Methanosarcina / genetics Nitrogen Fixation / genetics
CRISPR interference Methanosarcina archaea dCas9 gene regulation methanogens

Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
15 10 2020
Historique:
received: 15 06 2020
accepted: 15 08 2020
pubmed: 23 8 2020
medline: 2 12 2020
entrez: 23 8 2020
Statut: epublish

Résumé

CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The

Identifiants

DOI: 10.1128/AEM.01402-20 PMID: 32826220 PMC: PMC7580536
pubmed: 32826220
pii: AEM.01402-20
doi: 10.1128/AEM.01402-20
pmc: PMC7580536
pii:
doi:

Substances chimiques

Archaeal Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 American Society for Microbiology.

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Auteurs

Ahmed E Dhamad (AE)

Department of Biological Sciences, University of Arkansas-Fayetteville, Fayetteville, Arkansas, USA.
Department of Biological Sciences, Wasit University, Wasit, Iraq.
ORCID: 0000-0002-1846-5768

Daniel J Lessner (DJ)

Department of Biological Sciences, University of Arkansas-Fayetteville, Fayetteville, Arkansas, USA dlessner@uark.edu.
ORCID: 0000-0002-5323-8755

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'archée › A CRISPRi-dCas9 System for Archaea and Its Use...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › A CRISPRi-dCas9 System for Archaea and Its Use...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › A CRISPRi-dCas9 System for Archaea and Its Use...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome d'archéobactérie › Gènes d'archée › A CRISPRi-dCas9 System for Archaea and Its Use...
questionsmedicales.fr › Archéobactéries › Euryarchaeota › Methanosarcinales › Methanosarcinaceae › Methanosarcina › A CRISPRi-dCas9 System for Archaea and Its Use...
questionsmedicales.fr › Phénomènes biologiques › Phénomènes écologiques et environnementaux › Cycle de l'azote › Fixation de l'azote › A CRISPRi-dCas9 System for Archaea and Its Use...