Human herpesvirus 6A U27 plays an essential role for the virus propagation.
DNA replication
bacterial artificial chromosome
human herpesvirus 6
processivity factor
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
24
07
2020
revised:
18
08
2020
accepted:
19
08
2020
pubmed:
23
8
2020
medline:
9
6
2021
entrez:
23
8
2020
Statut:
ppublish
Résumé
Human herpesvirus 6A (HHV-6A) is a member of the genus Roseolovirus and the subfamily Betaherpesvirinae. It is similar to and human cytomegalovirus (HCMV). HHV-6A encodes a 41 kDa nuclear phosphoprotein, U27, which acts as a processivity factor in the replication of the viral DNA. HHV-6A U27 has 43% amino acid sequence homology with HCMV UL44, which is important for DNA replication. A previous study on HHV-6A U27 revealed that it greatly increases the in vitro DNA synthesis activity of HHV-6A DNA polymerase. However, the role of U27 during the HHV-6A virus replication process remains unclear. In this study, we constructed a U27-deficient HHV-6A mutant (HHV-6ABACU27mut) with a frameshift insertion at the U27 gene using an HHV-6A bacterial artificial chromosome (BAC) system. Viral reconstitution from the mutant BAC DNA was not detected, in contrast to the wild type and the revertant from the U27 mutant. This suggests that U27 plays a critical role in the life cycle of HHV-6A.
Identifiants
pubmed: 32827324
doi: 10.1111/1348-0421.12840
doi:
Substances chimiques
DNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
703-711Informations de copyright
© 2020 The Societies and John Wiley & Sons Australia, Ltd.
Références
Salahuddin SZ, Ablashi DV, Markham PD, et al. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986;234:596-601.
Okuno T, Takahashi K, Balachandra K, et al. Seroepidemiology of human herpesvirus 6 infection in normal children and adults. J Clin Microbiol. 1989;27:651-3.
Ablashi D, Agut H, Alvarez-Lafuente R, et al. Classification of HHV-6A and HHV-6B as distinct viruses. Arch Virol. 2014;159:863-70.
Gewurz BE, Marty FM, Baden LR, Katz JT. Human herpesvirus 6 encephalitis. Curr Infect Dis Rep. 2008;10:292-9.
Yamanishi K, Okuno T, Shiraki K, et al. Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet. 1988;1:1065-7.
Engdahl E, Gustafsson R, Huang J, et al. Increased serological response against human herpesvirus 6A is associated with risk for multiple sclerosis. Front Immunol. 2019;10:2715.
Virtanen JO, Farkkila M, Multanen J, et al. Evidence for human herpesvirus 6 variant A antibodies in multiple sclerosis: diagnostic and therapeutic implications. J Neurovirol. 2007;13:347-52.
Allnutt MA, Johnson K, Bennett DA, et al. Human herpesvirus 6 detection in Alzheimer's disease cases and controls across multiple cohorts. Neuron. 2020;105:1027-35.e2.
Bortolotti D, Gentili V, Rotola A, Caselli E, Rizzo R. HHV-6A infection induces amyloid-beta expression and activation of microglial cells. Alzheimers Res Ther. 2019;11:104.
Readhead B, Haure-Mirande JV, Ehrlich ME, Gandy S, Dudley JT. Clarifying the potential role of microbes in Alzheimer's disease. Neuron. 2019;104:1036-37.
Fremont M, Metzger K, Rady H, Hulstaert J, De Meirleir K. Detection of herpesviruses and parvovirus B19 in gastric and intestinal mucosa of chronic fatigue syndrome patients. In Vivo. 2009;23:209-13.
Broccolo F, Drago F, Cassina G, et al. Selective reactivation of human herpesvirus 6 in patients with autoimmune connective tissue diseases. J Med Virol. 2013;85:1925-34.
Ogata M, Fukuda T, Teshima T. Human herpesvirus-6 encephalitis after allogeneic hematopoietic cell transplantation: what we do and do not know. Bone Marrow Transplant. 2015;50:1030-6.
Gompels UA, Nicholas J, Lawrence G, et al. The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution. Virology. 1995;209:29-51.
Lin K, Ricciardi RP. The 41-kDa protein of human herpesvirus 6 specifically binds to viral DNA polymerase and greatly increases DNA synthesis. Virology. 1998;250:210-9.
Zhou Y, Chandran B, Wood C. Transcriptional patterns of the pCD41 (U27) locus of human herpesvirus 6. J Virol. 1997;71:3420-30.
Agulnick AD, Thompson JR, Iyengar S, Pearson G, Ablashi D, Ricciardi RP. Identification of a DNA-binding protein of human herpesvirus 6, a putative DNA polymerase stimulatory factor. J Gen Virol. 1993;74:1003-9.
Chang CK, Balachandran N. Identification, characterization, and sequence analysis of a cDNA encoding a phosphoprotein of human herpesvirus 6. J Virol. 1991;65:2884-94.
McGeoch DJ, Dalrymple MA, Dolan A, et al. Structures of herpes simplex virus type 1 genes required for replication of virus DNA. J Virol. 1988;62:444-53.
Gottlieb J, Challberg MD. Interaction of herpes simplex virus type 1 DNA polymerase and the UL42 accessory protein with a model primer template. J Virol. 1994;68:4937-45.
Reddig PJ, Grinstead LA, Monahan SJ, Johnson PA, Parris DS. The essential in vivo function of the herpes simplex virus UL42 protein correlates with its ability to stimulate the viral DNA polymerase in vitro. Virology. 1994;200:447-56.
Johnson PA, Best MG, Friedmann T, Parris DS. Isolation of a herpes simplex virus type 1 mutant deleted for the essential UL42 gene and characterization of its null phenotype. J Virol. 1991;65:700-10.
Weisshart K, Chow CS, Coen DM. Herpes simplex virus processivity factor UL42 imparts increased DNA-binding specificity to the viral DNA polymerase and decreased dissociation from primer-template without reducing the elongation rate. J Virol. 1999;73:55-66.
Cho MS, Milman G, Hayward SD. A second Epstein-Barr virus early antigen gene in BamHI fragment M encodes a 48- to 50-kilodalton nuclear protein. J Virol. 1985;56:860-6.
Neuhierl B, Delecluse HJ. The Epstein-Barr virus BMRF1 gene is essential for lytic virus replication. J Virol. 2006;80:5078-81.
Ertl PF, Powell KL. Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells. J Virol. 1992;66:4126-33.
Pari GS, Kacica MA, Anders DG. Open reading frames UL44, IRS1/TRS1, and UL36-38 are required for transient complementation of human cytomegalovirus oriLyt-dependent DNA synthesis. J Virol. 1993;67:2575-82.
Ripalti A, Boccuni MC, Campanini F, Landini MP. Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene. J Virol. 1995;69:2047-57.
Stasiak PC, Mocarski ES. Transactivation of the cytomegalovirus ICP36 gene promoter requires the alpha gene product TRS1 in addition to IE1 and IE2. J Virol. 1992;66:1050-8.
Dunn W, Chou C, Li H, et al. Functional profiling of a human cytomegalovirus genome. Proc Natl Acad Sci USA. 2003;100:14223-8.
Takeda K, Haque M, Nagoshi E, et al. Characterization of human herpesvirus 7 U27 gene product and identification of its nuclear localization signal. Virology. 2000;272:394-401.
Zarrouk K, Piret J, Boivin G. Herpesvirus DNA polymerases: structures, functions and inhibitors. Virus Res. 2017;234:177-92.
Tang HM, Kawabata A, Yoshida M, et al. Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth. Virology. 2010;407:360-367.
Dhepakson P, Mori Y, Jiang YB, et al. Human herpesvirus-6 rep/U94 gene product has single-stranded DNA-binding activity. J Gen Virol. 2002;83:847-54.
Akkapaiboon P, Mori Y, Sadaoka T, Yonemoto S, Yamanishi K. Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope. J Virol. 2004;78:7969-83.
Hayashi M, Yoshida K, Tang H, et al. Characterization of the human herpesvirus 6A U23 gene. Virology. 2014;450-451:98-105.
Mahmoud NF, Kawabata A, Tang H, et al. Human herpesvirus 6 U11 protein is critical for virus infection. Virology. 2016;489:151-7.
Niwa H, Yamamura K, Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991;108:193-9.
Oyaizu H, Tang H, Ota M, et al. Complementation of the function of glycoprotein H of human herpesvirus 6 variant A by glycoprotein H of variant B in the virus life cycle. J Virol. 2012;86:8492-8.
Tischer BK, von Einem J, Kaufer B, Osterrieder N. Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli. Biotechniques. 2006;40:191-7.
Jasirwan C, Tang H, Kawabata A, Mori Y. The human herpesvirus 6 U21-U24 gene cluster is dispensable for virus growth. Microbiol Immunol. 2015;59:48-53.
Appleton Brent A, Brooks J, Loregian A, Filman David J, Coen Donald M, Hogle James M, Crystal Structure of the Cytomegalovirus DNA Polymerase Subunit UL44 in Complex with the C Terminus from the Catalytic Subunit. J Biolog Chem. 2006;281:8:5224-32.
Fu YZ, Su S, Zou HM, et al. Human cytomegalovirus DNA polymerase subunit UL44 antagonizes antiviral immune responses by suppressing IRF3- and NF-kappaB-mediated transcription. J Virol. 2019;93:e00181-19.
Bonnafous P, Verbelen M, Petrella S, et al. Conservation of HHV-6 DNA polymerase processivity factor sequence and predicted structure suggests it as a target for antiviral development. Antiviral Res. 2010;86:316-9.
Madeira F, Park YM, Lee J, et al. The EMBL-EBI search and sequence analysis tools APIs in 2019. Nucleic Acids Res. 2019;47:W636-41.