Immunoinformatics-guided design of an epitope-based vaccine against severe acute respiratory syndrome coronavirus 2 spike glycoprotein.
Amino Acid Sequence
Antigens, Viral
/ chemistry
Betacoronavirus
/ genetics
COVID-19
COVID-19 Vaccines
Computational Biology
Coronavirus Infections
/ epidemiology
Drug Design
Epitopes, B-Lymphocyte
/ chemistry
Epitopes, T-Lymphocyte
/ chemistry
HLA-B15 Antigen
/ chemistry
HLA-DRB1 Chains
/ chemistry
Humans
Molecular Docking Simulation
Pandemics
/ prevention & control
Pneumonia, Viral
/ epidemiology
SARS-CoV-2
Spike Glycoprotein, Coronavirus
/ immunology
Viral Vaccines
/ chemistry
COVID-19
Epitope
Immunoinformatics
SARS-CoV-2
Spike glycoprotein
Journal
Computers in biology and medicine
ISSN: 1879-0534
Titre abrégé: Comput Biol Med
Pays: United States
ID NLM: 1250250
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
28
05
2020
revised:
08
08
2020
accepted:
08
08
2020
pubmed:
23
8
2020
medline:
25
9
2020
entrez:
23
8
2020
Statut:
ppublish
Résumé
With a large number of fatalities, coronavirus disease-2019 (COVID-19) has greatly affected human health worldwide. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19. The World Health Organization has declared a global pandemic of this contagious disease. Researchers across the world are collaborating in a quest for remedies to combat this deadly virus. It has recently been demonstrated that the spike glycoprotein (SGP) of SARS-CoV-2 is the mediator by which the virus enters host cells. Our group comprehensibly analyzed the SGP of SARS-CoV-2 through multiple sequence analysis and a phylogenetic analysis. We predicted the strongest immunogenic epitopes of the SGP for both B cells and T cells. We focused on predicting peptides that would bind major histocompatibility complex class I. Two optimal epitopes were identified, WTAGAAAYY and GAAAYYVGY. They interact with the HLA-B*15:01 allele, which was further validated by molecular docking simulation. This study also found that the selected epitopes are able to be recognized in a large percentage of the world's population. Furthermore, we predicted CD4 Our study provides a strong basis for designing vaccine candidates against SARS-CoV-2. However, laboratory work is required to validate our theoretical results, which would lay the foundation for the appropriate vaccine manufacturing and testing processes.
Identifiants
pubmed: 32828069
pii: S0010-4825(20)30300-0
doi: 10.1016/j.compbiomed.2020.103967
pmc: PMC7423576
pii:
doi:
Substances chimiques
Antigens, Viral
0
COVID-19 Vaccines
0
Epitopes, B-Lymphocyte
0
Epitopes, T-Lymphocyte
0
HLA-B15 Antigen
0
HLA-DRB1 Chains
0
Spike Glycoprotein, Coronavirus
0
Viral Vaccines
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103967Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
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