Toxicological implication of prostaglandin transporter SLCO2A1 inhibition by cigarette smoke in exacerbation of lung inflammation.
Animals
Bleomycin
/ toxicity
Bronchoalveolar Lavage Fluid
/ chemistry
Cytokines
/ genetics
Dinoprostone
/ metabolism
Disease Models, Animal
Female
Gene Expression
/ drug effects
HEK293 Cells
Humans
Lung
/ drug effects
Male
Mice
Mice, Knockout
Organic Anion Transporters
/ genetics
Pulmonary Fibrosis
/ chemically induced
Smoking
/ adverse effects
Tobacco Products
/ toxicity
Tobacco Smoke Pollution
/ adverse effects
Cigarette Smoke
Fibrosis
Inflammation
Lung
Prostaglandin
SLCO2A1
Journal
Toxicology and applied pharmacology
ISSN: 1096-0333
Titre abrégé: Toxicol Appl Pharmacol
Pays: United States
ID NLM: 0416575
Informations de publication
Date de publication:
15 10 2020
15 10 2020
Historique:
received:
24
05
2020
revised:
11
08
2020
accepted:
14
08
2020
pubmed:
24
8
2020
medline:
12
1
2021
entrez:
24
8
2020
Statut:
ppublish
Résumé
We reported that bleomycin (BLM)-induced pulmonary fibrosis was exacerbated in the prostaglandin transporter gene (Slco2a1)-deficient mice (Slco2a1(-/-)). Because cigarette smoke (CS) contributes to creating a profibrotic milieu in the respiratory region, the present study aimed to investigate the impact of CS on SLCO2A1-associated pathogenesis in the lungs of BLM-instilled mice. Bronchoalveolar lavage (BAL) fluid cell analysis indicated more severe inflammation in Slco2a1(-/-) on day 5 after BLM intratracheal instillation, and Slco2a1 deletion increased mRNA expression of pro-inflammatory cytokines (Tnf-α and Il-1β) and chemokine (Ccl5) in BAL cells. Male Slco2a1(-/-) exhibited significantly higher amounts of released Il-1β in BAL fluid, compared with female Slco2a1(-/-), male or female Slco2a1(+/+) group. The amount of PGE
Identifiants
pubmed: 32828905
pii: S0041-008X(20)30327-6
doi: 10.1016/j.taap.2020.115201
pii:
doi:
Substances chimiques
Cytokines
0
Organic Anion Transporters
0
Slco2a1 protein, mouse
0
Tobacco Smoke Pollution
0
Bleomycin
11056-06-7
Dinoprostone
K7Q1JQR04M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
115201Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest There is no conflict of interest to report.