Plasma cell-free DNA methylation marks for episodic memory impairment: a pilot twin study.
Aged
Alzheimer Disease
/ diagnosis
Cell-Free Nucleic Acids
/ blood
Cognition
/ physiology
Cognitive Dysfunction
/ genetics
DNA Methylation
/ physiology
Female
Finland
Genome-Wide Association Study
Humans
Male
Memory
/ physiology
Memory Disorders
/ genetics
Memory, Episodic
Neuropsychological Tests
Plasma
Twins
/ genetics
Twins, Monozygotic
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 08 2020
25 08 2020
Historique:
received:
16
03
2020
accepted:
05
08
2020
entrez:
27
8
2020
pubmed:
28
8
2020
medline:
12
1
2021
Statut:
epublish
Résumé
Decline in episodic memory performance usually causes the first clinical symptoms of Alzheimer's disease. At present, Alzheimer's disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed for earlier and more efficient Alzheimer's disease intervention. To identify early disease markers, we implemented a genome-wide bisulphite sequencing method for the analysis of plasma cell-free DNA methylation profiles and compared differences associated with episodic memory performance in Finnish twin pairs. A noticeable amount of cell-free DNA was present in plasma, however, the amounts as well as the genomic coverage of these fragments varied substantially between individuals. We found no significant markers associated with episodic memory performance in the twins' plasma cell-free DNA methylation profiles. Furthermore, our results indicate that due to the low genomic coverage of cell-free DNA fragments and the variety in these fragments between individuals, the implemented genome-wide bisulphite sequencing method is not optimal for comparing cell-free DNA methylation differences between large groups of individuals.
Identifiants
pubmed: 32843700
doi: 10.1038/s41598-020-71239-9
pii: 10.1038/s41598-020-71239-9
pmc: PMC7447764
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14192Références
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