Characterizing the transport and utilization of the neurotransmitter GABA in the bacterial pathogen Brucella abortus.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
04
2020
accepted:
04
08
2020
entrez:
27
8
2020
pubmed:
28
8
2020
medline:
21
10
2020
Statut:
epublish
Résumé
The neurotransmitter gamma-aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the human brain; however, it is becoming more evident that this non-proteinogenic amino acid plays multiple physiological roles in biology. In the present study, the transport and function of GABA is studied in the highly infectious intracellular bacterium Brucella abortus. The data show that 3H-GABA is imported by B. abortus under nutrient limiting conditions and that the small RNAs AbcR1 and AbcR2 negatively regulate this transport. A specific transport system, gts, is responsible for the transport of GABA as determined by measuring 3H-GABA transport in isogenic deletion strains of known AbcR1/2 regulatory targets; however, this locus is unnecessary for Brucella infection in BALB/c mice. Similar assays revealed that 3H-GABA transport is uninhibited by the 20 standard proteinogenic amino acids, representing preference for the transport of 3H-GABA. Metabolic studies did not show any potential metabolic utilization of GABA by B. abortus as a carbon or nitrogen source, and RNA sequencing analysis revealed limited transcriptional differences between B. abortus 2308 with or without exposure to GABA. While this study provides evidence for GABA transport by B. abortus, questions remain as to why and when this transport is utilized during Brucella pathogenesis.
Identifiants
pubmed: 32845904
doi: 10.1371/journal.pone.0237371
pii: PONE-D-20-12100
pmc: PMC7449393
doi:
Substances chimiques
Neurotransmitter Agents
0
Glutamic Acid
3KX376GY7L
gamma-Aminobutyric Acid
56-12-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0237371Subventions
Organisme : NIAID NIH HHS
ID : R21 AI125958
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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