EPR Spectroscopy as a Method for ROS Quantification in the Skin.
Cumulative radical production
Electron paramagnetic resonance (EPR) spectroscopy
Free radicals
Skin
Spin probe
UVA light
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
29
8
2020
pubmed:
29
8
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.
Identifiants
pubmed: 32857353
doi: 10.1007/978-1-0716-0896-8_12
doi:
Substances chimiques
Cyclic N-Oxides
0
Free Radicals
0
Reactive Oxygen Species
0
Spin Labels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM