CRISPR screens in the era of microbiomes.

Animals Bacteria / classification Bacterial Infections / microbiology Clustered Regularly Interspaced Short Palindromic Repeats Genomics Host-Pathogen Interactions Humans Microbiota

Journal

Current opinion in microbiology
ISSN: 1879-0364
Titre abrégé: Curr Opin Microbiol
Pays: England
ID NLM: 9815056

Informations de publication

Date de publication:
10 2020
Historique:
received: 10 02 2020
revised: 13 07 2020
accepted: 17 07 2020
pubmed: 29 8 2020
medline: 1 6 2021
entrez: 29 8 2020
Statut: ppublish

Résumé

Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.

Identifiants

DOI: 10.1016/j.mib.2020.07.009 PMID: 32858412
pubmed: 32858412
pii: S1369-5274(20)30090-4
doi: 10.1016/j.mib.2020.07.009
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

70-77

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Auteurs

François Rousset (F)

Synthetic Biology Group, Microbiology Department, Institut Pasteur, Paris, France; Sorbonne Université, Collège Doctoral, F-75005 Paris, France.

David Bikard (D)

Synthetic Biology Group, Microbiology Department, Institut Pasteur, Paris, France. Electronic address: david.bikard@pasteur.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Bactéries › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › CRISPR screens in the era of microbiomes.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › CRISPR screens in the era of microbiomes.