The T-type calcium channel isoform Ca
calcium channel
electrophysiology
hypnosis
mechanisms of anaesthesia
neurosteroid
thalamus
Journal
British journal of anaesthesia
ISSN: 1471-6771
Titre abrégé: Br J Anaesth
Pays: England
ID NLM: 0372541
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
29
10
2019
revised:
02
07
2020
accepted:
08
07
2020
pubmed:
30
8
2020
medline:
29
1
2021
entrez:
30
8
2020
Statut:
ppublish
Résumé
The mechanisms underlying the role of T-type calcium channels (T-channels) in thalamocortical excitability and oscillations in vivo during neurosteroid-induced hypnosis are largely unknown. We used patch-clamp electrophysiological recordings from acute brain slices ex vivo, recordings of local field potentials (LFPs) from the central medial thalamic nucleus in vivo, and wild-type (WT) and Ca Patch-clamp recordings showed that 3β-OH inhibited isolated T-currents but had no effect on phasic or tonic γ-aminobutyric acid A currents. Also in acute brain slices, 3β-OH inhibited the spike firing mode more profoundly in WT than in Ca The Ca
Sections du résumé
BACKGROUND
The mechanisms underlying the role of T-type calcium channels (T-channels) in thalamocortical excitability and oscillations in vivo during neurosteroid-induced hypnosis are largely unknown.
METHODS
We used patch-clamp electrophysiological recordings from acute brain slices ex vivo, recordings of local field potentials (LFPs) from the central medial thalamic nucleus in vivo, and wild-type (WT) and Ca
RESULTS
Patch-clamp recordings showed that 3β-OH inhibited isolated T-currents but had no effect on phasic or tonic γ-aminobutyric acid A currents. Also in acute brain slices, 3β-OH inhibited the spike firing mode more profoundly in WT than in Ca
CONCLUSIONS
The Ca
Identifiants
pubmed: 32859366
pii: S0007-0912(20)30611-5
doi: 10.1016/j.bja.2020.07.022
pmc: PMC7844375
pii:
doi:
Substances chimiques
3-hydroxyandrostane-17-carbonitrile
0
Androstanols
0
Calcium Channels, T-Type
0
Hypnotics and Sedatives
0
Neurosteroids
0
Nitriles
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-255Subventions
Organisme : NINDS NIH HHS
ID : P30 NS048154
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123746
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS086839
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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