Underlying molecular mechanism in the modulation of the ram sperm acrosome reaction by progesterone and 17β-estradiol.
Acrosome reaction
Ca(2+) mobilization
ROS
Spermatozoa
Steroid hormones
Tyrosine-phosphorylation
Journal
Animal reproduction science
ISSN: 1873-2232
Titre abrégé: Anim Reprod Sci
Pays: Netherlands
ID NLM: 7807205
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
31
03
2020
revised:
23
07
2020
accepted:
24
07
2020
pubmed:
30
8
2020
medline:
31
7
2021
entrez:
30
8
2020
Statut:
ppublish
Résumé
Steroid hormones progesterone (P4) and 17β-estradiol (E2) not only have important functions in regulation of reproductive processes in mammals but also have direct effects on spermatozoa. There can be induction of the acrosome reaction in ram spermatozoa by P4 and E2 and, in the present study, there was further investigation of mechanisms underlying this effect. In a medium containing agents that increase cAMP, the presence of both P4 and E2 led to changes in the localization of proteins phosphorylated in tyrosine residues evaluated by indirect immunofluorescence. The inclusion of P4 at 1 μM in the media induced an increase in Ca
Identifiants
pubmed: 32861117
pii: S0378-4320(20)30439-5
doi: 10.1016/j.anireprosci.2020.106567
pii:
doi:
Substances chimiques
Estrogens
0
Progestins
0
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106567Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.