Ultrasensitive colorimetric biosensor for BRCA1 mutation based on multiple signal amplification strategy.

Biosensing Techniques Colorimetry Gold Limit of Detection Metal Nanoparticles Mutation Reproducibility of Results
2D nanomaterials BRCA1 Bismuth selenide nanosheets Colorimetric biosensor Gold nanoparticles Signal amplification

Journal

Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289

Informations de publication

Date de publication:
15 Oct 2020
Historique:
received: 22 04 2020
revised: 15 06 2020
accepted: 02 07 2020
entrez: 1 9 2020
pubmed: 31 8 2020
medline: 15 5 2021
Statut: ppublish

Résumé

Colorimetric biosensors have attracted wide attention due to their low cost, simple operation, rapid response and good reproducibility. However, insufficient sensitivity limits their applications. This report describes the design of a colorimetric biosensor based on a three-step multiple signal amplification strategy to detect breast cancer-associated BRCA1 mutation. The capture unit, signal unit, and target DNA form a sandwich construction. The signal probes are immobilized on the surface of nanomaterials to form the signal unit, which can catalyze the reduction of a colorimetric substrate 4-nitrophenol (4-NP). Firstly, 0D gold nanoparticles (AuNPs) are employed to catalyze 4-NP reduction and reaches 10

Identifiants

DOI: 10.1016/j.bios.2020.112424 PMID: 32862841
pubmed: 32862841
pii: S0956-5663(20)30418-8
doi: 10.1016/j.bios.2020.112424
pii:
doi:

Substances chimiques

Gold 7440-57-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

112424

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Yunfei Bai (Y)

Key Laboratory of Biomedical Engineering of Fujian Province, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China.

Huizhen Li (H)

Key Laboratory of Biomedical Engineering of Fujian Province, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China.

Jun Xu (J)

Department of Physics, Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials, Xiamen University, Xiamen, 361005, China.

Yufan Huang (Y)

Department of Breast Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China.

Xiuming Zhang (X)

Key Laboratory of Biomedical Engineering of Fujian Province, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China.

Jian Weng (J)

Key Laboratory of Biomedical Engineering of Fujian Province, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China. Electronic address: jweng@xmu.edu.cn.

Zhu Li (Z)

Xiamen Nuokangde Biological Technology Co., Ltd, Xiamen, 361006, China. Electronic address: lizhu@nkdbio.com.

Liping Sun (L)

Key Laboratory of Biomedical Engineering of Fujian Province, Department of Biomaterials, College of Materials, Xiamen University, Xiamen, 361005, China. Electronic address: sunliping@xmu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Titrimétrie › Colorimétrie › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Or › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Nanoparticules métalliques › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Ultrasensitive colorimetric biosensor for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Ultrasensitive colorimetric biosensor for...