Blood loss induces l-amino acid oxidase gene expression in the head kidney of the red-spotted grouper, Epinephelus akaara.
Antibacterial protein
Blood loss response
Broad substrate specificity
Epinephelus akaara
l-amino acid oxidase (LAO)
Journal
Developmental and comparative immunology
ISSN: 1879-0089
Titre abrégé: Dev Comp Immunol
Pays: United States
ID NLM: 7708205
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
12
06
2020
revised:
03
08
2020
accepted:
24
08
2020
pubmed:
1
9
2020
medline:
10
2
2022
entrez:
1
9
2020
Statut:
ppublish
Résumé
In fish, the innate immune system is more important than the adaptive immune system because it responds quickly and nonspecifically to protect against pathogens. Thus, a variety of innate immune molecules have been found in fish. Recently, l-amino acid oxidases (LAOs) were discovered as a new member of the antibacterial protein from fish skin mucus and serum. In this study, we newly found an antibacterial LAO in red-spotted grouper (Epinephelus akaara) serum. It showed a broad range of substrate specificity with aromatic and hydrophobic amino acids. The grouper LAO gene had a low expression level in the kidney under normal conditions; however, it was significantly upregulated by blood loss 1 day after bleeding. In addition, the LAO activity in the serum recovered within 3 days in the same experiment. This quick recovery may indicate that the LAO is an essential innate immune molecule in the whole grouper body.
Identifiants
pubmed: 32866581
pii: S0145-305X(20)30397-9
doi: 10.1016/j.dci.2020.103842
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Fish Proteins
0
L-Amino Acid Oxidase
EC 1.4.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103842Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.