Effect of total flavonoids of Rhizoma Drynariae in thiram induced cytotoxicity of chondrocyte via BMP-2/Runx2 and IHH/PTHrP expressions.
Animals
Bone Morphogenetic Protein 2
/ genetics
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cells, Cultured
Chickens
Chondrocytes
/ drug effects
Core Binding Factor Alpha 1 Subunit
/ genetics
Flavonoids
/ isolation & purification
Gene Expression
/ drug effects
Growth Plate
/ cytology
Hedgehog Proteins
/ genetics
Parathyroid Hormone-Related Protein
/ genetics
Polypodiaceae
/ chemistry
Primary Cell Culture
Rhizome
Thiram
/ toxicity
Up-Regulation
BMP-2/Runx2
Chondrocyte
IHH/PTHrP
Rhizoma drynariae
Tibial dyschondroplasia
Journal
Ecotoxicology and environmental safety
ISSN: 1090-2414
Titre abrégé: Ecotoxicol Environ Saf
Pays: Netherlands
ID NLM: 7805381
Informations de publication
Date de publication:
15 Dec 2020
15 Dec 2020
Historique:
received:
11
07
2020
revised:
14
08
2020
accepted:
17
08
2020
pubmed:
1
9
2020
medline:
2
12
2020
entrez:
1
9
2020
Statut:
ppublish
Résumé
Tibial Dyschondroplasia (TD) is a prevailing skeletal disorder that mainly affects rapidly growing avian species. It results in reduced bone strength, lameness and an increase risk of fragility fractures. Total flavonoids of Rhizoma drynariae (TFRD) have been used as an effective treatment of different bone diseases in humans. The current in vitro study was conducted to explore the therapeutic effect of TFRD on thiram-induced cytotoxicity in avian growth plate cells via bone morphogenetic protein-2/runt related transcription factor-2 (BMP-2/Runx2) and Indian hedgehog/Parathyroid hormone-related peptide (IHH/PTHrP) expressions. Chondrocytes were isolated, cultured and refined from chicken's tibial growth plates in a special medium. Then chondrocytes were treated with sublethal thiram having less concentration (2.5 μg/mL) to induce cytotoxicity of chondrocyte, and then treated with providential doses (100 μg/mL) of TFRD. Thiram caused distorted morphology of chondrocytes, nuclei appeared disintegration or lysed along with decreased expressions of BMP-2/Runx2 and IHH/PTHrP. TFRD administration not only enhanced the viability of chondrocytes by itself, but also well restored the damage caused by thiram on growth plate chondrocytes by significantly up-regulating the expressions of BMP-2/Runx2 and IHH/PTHrP. Therefore, this study provides a novel insight into the further treatment of TD and other skeletal ailments and lays the foundation for prevention and treatment.
Identifiants
pubmed: 32866894
pii: S0147-6513(20)31033-2
doi: 10.1016/j.ecoenv.2020.111194
pii:
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Core Binding Factor Alpha 1 Subunit
0
Flavonoids
0
Hedgehog Proteins
0
Parathyroid Hormone-Related Protein
0
Thiram
0D771IS0FH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111194Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.