[Application of unrestrained conscious rats with acute inflammatory ankle pain to study of acupuncture analgesia].

To compare the analgesic effect of manual acupuncture(MA) stimulation of "Zusanli" (ST36) in rats with inflammatory pain under unrestrained conscious, restrained and general anesthesia conditions, so as to explore the applicability of unrestrained conscious model in the evaluation of acupuncture analgesia effect. Male SD rats were divided into 5 groups: blank control ( Both MPT and TPT were significantly decreased after injection of CFA in the model group relevant to the blank control group ( MA has a better therapeutic effect in relieving pain and pain-induced depression-like behavior in conscious unrestrained rats than in restrained and GA rats, implying a higher applicability of unrestrained conscious pain model to the assessment of acupuncture analgesia.