Extensive and spatially variable within-cell-type heterogeneity across the basolateral amygdala.
amygdala
cell type
mouse
neural circuits
neuroscience
transcriptomics
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
16
05
2020
accepted:
26
08
2020
pubmed:
2
9
2020
medline:
12
2
2021
entrez:
2
9
2020
Statut:
epublish
Résumé
The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization, we next mapped this discrete transcriptomic heterogeneity onto a sharp spatial border between the basal and lateral amygdala nuclei, and identified continuous spatial gene-expression gradients within each of these regions. These discrete and continuous spatial transformations of transcriptomic cell-type identity were recapitulated by local morphology as well as long-range connectivity. Thus, BLA excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties. This heterogeneity likely drives pronounced spatial variation in BLA computation and function.
Identifiants
pubmed: 32869744
doi: 10.7554/eLife.59003
pii: 59003
pmc: PMC7486123
doi:
pii:
Banques de données
GEO
['GSE148866']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN-2019-04507
Pays : International
Organisme : CIHR
ID : PJT-419798
Pays : Canada
Organisme : Canadian Foundation for Innovation
ID : 38369
Pays : International
Organisme : Michael Smith Foundation for Health Research
ID : SCH-2020-0383
Pays : International
Informations de copyright
© 2020, O'Leary et al.
Déclaration de conflit d'intérêts
TO, KS, LW, JC, AL, MC No competing interests declared
Références
Ann N Y Acad Sci. 2003 Apr;985:185-205
pubmed: 12724159
Nat Neurosci. 2016 Oct;19(10):1348-55
pubmed: 27595384
Elife. 2018 Oct 30;7:
pubmed: 30375971
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
Nature. 2011 Mar 17;471(7338):358-62
pubmed: 21389985
Learn Mem. 2001 May-Jun;8(3):148-55
pubmed: 11390634
Neuron. 2017 Oct 11;96(2):313-329.e6
pubmed: 29024657
Nat Neurosci. 2016 Dec;19(12):1636-1646
pubmed: 27749826
Neuron. 1999 Jun;23(2):229-32
pubmed: 10399930
Neurosci Biobehav Rev. 2015 Oct;57:271-83
pubmed: 26341938
Neuron. 2016 Apr 20;90(2):348-361
pubmed: 27041499
Neuron. 2019 May 8;102(3):636-652.e7
pubmed: 30905392
Trends Neurosci. 1998 Aug;21(8):323-31
pubmed: 9720596
Neuron. 2020 Jul 22;107(2):274-282.e6
pubmed: 32396852
Neuron. 2018 Sep 19;99(6):1315-1328.e5
pubmed: 30146300
Neuron. 2018 Feb 7;97(3):656-669.e7
pubmed: 29420934
Nature. 2007 Jan 11;445(7124):168-76
pubmed: 17151600
Nat Neurosci. 2017 Jun;20(6):824-835
pubmed: 28436980
Neuron. 2020 Feb 19;105(4):688-699.e8
pubmed: 31813651
Nat Rev Neurosci. 2004 Nov;5(11):844-52
pubmed: 15496862
Cell. 2018 Aug 9;174(4):999-1014.e22
pubmed: 30096314
Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5270-5
pubmed: 11320257
Nat Rev Neurosci. 2017 Sep;18(9):530-546
pubmed: 28775344
Nat Rev Neurosci. 2015 Jun;16(6):317-31
pubmed: 25991441
Brain Struct Funct. 2013 Nov;218(6):1569-89
pubmed: 23179863
Neuron. 2017 May 17;94(4):747-751.e1
pubmed: 28521129
Neuron. 2016 Oct 19;92(2):372-382
pubmed: 27720486
Cell Rep. 2017 Nov 7;21(6):1426-1433
pubmed: 29117549
Physiol Rev. 2003 Jul;83(3):803-34
pubmed: 12843409
Handb Behav Neurosci. 2020;26:63-100
pubmed: 32792868
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Neuron. 2014 Jan 22;81(2):428-37
pubmed: 24462103
Cell. 2018 May 17;173(5):1280-1292.e18
pubmed: 29681453
Nat Rev Neurosci. 2019 Apr;20(4):193-204
pubmed: 30778192
Brain Struct Funct. 2016 Jul;221(6):2937-62
pubmed: 26169110
Nature. 2018 Nov;563(7729):79-84
pubmed: 30382200
J Comp Neurol. 1984 Feb 1;222(4):589-606
pubmed: 6199387
Nat Biotechnol. 2018 Dec 03;:
pubmed: 30531897
Neuron. 2013 Aug 21;79(4):658-64
pubmed: 23972595
Neurobiol Stress. 2019 Aug 13;11:100191
pubmed: 31467945
Sci Rep. 2019 Dec 23;9(1):19610
pubmed: 31873139
Bioinformatics. 2009 May 1;25(9):1105-11
pubmed: 19289445
J Neurosci. 2014 Jan 8;34(2):586-95
pubmed: 24403157
Neuron. 2016 Jan 20;89(2):351-68
pubmed: 26777276
BMC Bioinformatics. 2008 Mar 18;9:153
pubmed: 18366675
Science. 2017 Oct 6;358(6359):64-69
pubmed: 28983044
J Neurosci. 2013 Sep 25;33(39):15333-42
pubmed: 24068800
Curr Biol. 2007 Oct 23;17(20):R868-74
pubmed: 17956742
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Nature. 2008 Jul 31;454(7204):600-6
pubmed: 18615015
Cell Rep. 2018 Jan 23;22(4):905-918
pubmed: 29386133
Annu Rev Neurosci. 2017 Jul 25;40:629-652
pubmed: 28661727
J Neurosci Methods. 2019 Oct 1;326:108353
pubmed: 31351971
Brain Struct Funct. 2007 Sep;212(2):149-79
pubmed: 17717690
Nature. 2014 Apr 10;508(7495):207-14
pubmed: 24695228
Nature. 2015 Jan 15;517(7534):284-92
pubmed: 25592533
Nature. 2013 Apr 11;496(7444):219-23
pubmed: 23515158
Nature. 2015 Apr 30;520(7549):675-8
pubmed: 25925480
Nat Protoc. 2007;2(11):2924-9
pubmed: 18007629
Nat Neurosci. 2019 Nov;22(11):1925-1935
pubmed: 31527803
Neuron. 2016 Feb 17;89(4):683-94
pubmed: 26889809
Trends Neurosci. 2018 Jun;41(6):337-348
pubmed: 29576429
Nat Rev Neurosci. 2002 Jul;3(7):563-73
pubmed: 12094212
Cell Rep. 2020 Apr 28;31(4):107551
pubmed: 32348756
Neuroscience. 2016 May 3;321:197-209
pubmed: 26204817
Nat Biotechnol. 2015 May;33(5):495-502
pubmed: 25867923
PLoS Biol. 2018 Jun 18;16(6):e2006387
pubmed: 29912866