Long non-coding RNA LINC00858 aggravates the oncogenic phenotypes of ovarian cancer cells through miR-134-5p/RAD18 signaling.
Apoptosis
Carcinogenesis
/ genetics
Carcinoma, Ovarian Epithelial
/ genetics
Cell Movement
/ genetics
Cell Proliferation
/ genetics
DNA-Binding Proteins
Epithelial-Mesenchymal Transition
/ genetics
Female
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs
/ genetics
Ovarian Neoplasms
/ genetics
RNA, Long Noncoding
/ genetics
Real-Time Polymerase Chain Reaction
Signal Transduction
Ubiquitin-Protein Ligases
/ genetics
Up-Regulation
LINC00858
Ovarian cancer
RAD18
miR-134-5p
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
19
03
2020
accepted:
28
07
2020
pubmed:
3
9
2020
medline:
16
12
2020
entrez:
3
9
2020
Statut:
ppublish
Résumé
Ovarian cancer is a common gynecological cancer. Herein, we focused on the function and probable mechanisms of LINC00858 in ovarian cancer. Real-time quantitative polymerase chain reaction (RT-qPCR) was employed for detecting the expression of LINC00858, miR-134-5p and RAD18 E3 ubiquitin protein ligase (RAD18). Cell proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) and apoptosis were detected by cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), transwell, terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) and western bolt experiments, as appropriate. Interplays between LINC00858, miR-134-5p and RAD18 were detected by RNA immunoprecipitation (RIP), RNA pull down and luciferase reporter assays. LINC00858 were up-regulated in ovarian cancer tissues and cells, and its expression was elevated in advanced samples compared to early ones. Knocking down LINC00858 inhibited cell proliferation, motility and EMT, but accelerated cell apoptosis in ovarian cancer. Moreover, could be sponged by LINC00858 sponged miR-134-5p to enhance RAD18 expression in ovarian cancer. Also, silenced RAD18 could also restrain oncogenic behaviors of ovarian cancer cells. Rescue experiments showed that overexpressing RAD18 reversed the effects caused by knocking down LINC00858 on cellular processes. LINC00858 sequestered miR-134-5p to elevate RAD18 expression, resulting in aggravated development of ovarian cancer. This might provide promising targets for treating patients with ovarian cancer.
Identifiants
pubmed: 32875345
doi: 10.1007/s00404-020-05722-z
pii: 10.1007/s00404-020-05722-z
doi:
Substances chimiques
DNA-Binding Proteins
0
MicroRNAs
0
RAD18 protein, human
0
RNA, Long Noncoding
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1243-1254Références
Siegel RL, Miller KD, Jemal A (2017) Cancer statistics, 2017. CA: Cancer J Clin 67(1):7–30. https://doi.org/10.3322/caac.21387
doi: 10.3322/caac.21387
Rottmann M, Burges A, Mahner S, Anthuber C, Beck T, Grab D, Schnelzer A, Kiechle M, Mayr D, Polcher M, Schubert-Fritschle G, Engel J (2017) Cancer of the ovary, fallopian tube, and peritoneum: a population-based comparison of the prognostic factors and outcomes. J Cancer Res Clin Oncol 143(9):1833–1844. https://doi.org/10.1007/s00432-017-2422-6
doi: 10.1007/s00432-017-2422-6
pubmed: 28447160
Kujawa KA, Lisowska KM (2015) Ovarian cancer–from biology to clinic. Postepy Hig Med Dosw(Online) 69:1275–1290. https://doi.org/10.5604/17322693.1184451
doi: 10.5604/17322693.1184451
Eisenhauer EA (2017) Real-world evidence in the treatment of ovarian cancer. Ann Oncol 28((suppl_8)):viii61–viii65. https://doi.org/10.1093/annonc/mdx443
doi: 10.1093/annonc/mdx443
pubmed: 29232466
Hoskins PJ, Gotlieb WH (2017) Missed therapeutic and prevention opportunities in women with BRCA-mutated epithelial ovarian cancer and their families due to low referral rates for genetic counseling and BRCA testing: a review of the literature. CA: Cancer J Clini 67(6):493–506. https://doi.org/10.3322/caac.21408
doi: 10.3322/caac.21408
Bhan A, Soleimani M, Mandal SS (2017) Long noncoding RNA and cancer: a new paradigm. Can Res 77(15):3965–3981. https://doi.org/10.1158/0008-5472.Can-16-2634
doi: 10.1158/0008-5472.Can-16-2634
Peng WX, Koirala P, Mo YY (2017) LncRNA-mediated regulation of cell signaling in cancer. Oncogene 36(41):5661–5667. https://doi.org/10.1038/onc.2017.184
doi: 10.1038/onc.2017.184
pubmed: 28604750
pmcid: 6450570
Beermann J, Piccoli MT, Viereck J, Thum T (2016) Non-coding RNAs in development and disease: background, mechanisms, and therapeutic approaches. Physiol Rev 96(4):1297–1325. https://doi.org/10.1152/physrev.00041.2015
doi: 10.1152/physrev.00041.2015
Zhao Z, Zhou W, Han Y, Peng F, Wang R, Yu R, Wang C, Liang H, Guo Z, Gu Y (2017) EMT-Regulome: a database for EMT-related regulatory interactions, motifs and network. Cell Death Dis 8(6):e2872. https://doi.org/10.1038/cddis.2017.267
doi: 10.1038/cddis.2017.267
pubmed: 28617437
pmcid: 5520922
Bach DH, Lee SK (2018) Long noncoding RNAs in cancer cells. Cancer Lett 419:152–166. https://doi.org/10.1016/j.canlet.2018.01.053
doi: 10.1016/j.canlet.2018.01.053
pubmed: 29414303
Lai Y, Chen Y, Lin Y, Ye L (2018) Down-regulation of LncRNA CCAT1 enhances radiosensitivity via regulating miR-148b in breast cancer. Cell Biol Int 42(2):227–236. https://doi.org/10.1002/cbin.10890
doi: 10.1002/cbin.10890
pubmed: 29024383
Li Z, Hong S, Liu Z (2018) LncRNA LINC00641 predicts prognosis and inhibits bladder cancer progression through miR-197-3p/KLF10/PTEN/PI3K/AKT cascade. Biochem Biophys Res Commun 503(3):1825–1829. https://doi.org/10.1016/j.bbrc.2018.07.120
doi: 10.1016/j.bbrc.2018.07.120
pubmed: 30060954
Luan X, Wang Y (2018) LncRNA XLOC_006390 facilitates cervical cancer tumorigenesis and metastasis as a ceRNA against miR-331-3p and miR-338-3p. J Gynecol Oncol 29(6):e95. https://doi.org/10.3802/jgo.2018.29.e95
doi: 10.3802/jgo.2018.29.e95
pubmed: 30207103
pmcid: 6189437
Du W, Feng Z, Sun Q (2018) LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway. Biochem Biophys Res Commun 507(1–4):198–202. https://doi.org/10.1016/j.bbrc.2018.11.006
doi: 10.1016/j.bbrc.2018.11.006
pubmed: 30442370
Sha QK, Chen L, Xi JZ, Song H (2019) Long non-coding RNA LINC00858 promotes cells proliferation, migration and invasion by acting as a ceRNA of miR-22-3p in colorectal cancer. Artif Cells Nanomed Biotechnol 47(1):1057–1066. https://doi.org/10.1080/21691401.2018.1544143
doi: 10.1080/21691401.2018.1544143
pubmed: 30931636
Gu Z, Hou Z, Zheng L, Wang X, Wu L, Zhang C (2018) Long noncoding RNA LINC00858 promotes osteosarcoma through regulating miR-139-CDK14 axis. Biochem Biophys Res Commun 503(2):1134–1140. https://doi.org/10.1016/j.bbrc.2018.06.131
doi: 10.1016/j.bbrc.2018.06.131
pubmed: 29944887
Liu Y, Beyer A, Aebersold R (2016) On the dependency of cellular protein levels on mRNA abundance. Cell 165(3):535–550. https://doi.org/10.1016/j.cell.2016.03.014
doi: 10.1016/j.cell.2016.03.014
pubmed: 27104977
pmcid: 27104977
Tien JF, Mazloomian A, Cheng SG, Hughes CS, Chow CCT, Canapi LT, Oloumi A, Trigo-Gonzalez G, Bashashati A, Xu J, Chang VC, Shah SP, Aparicio S, Morin GB (2017) CDK12 regulates alternative last exon mRNA splicing and promotes breast cancer cell invasion. Nucleic Acids Res 45(11):6698–6716. https://doi.org/10.1093/nar/gkx187
doi: 10.1093/nar/gkx187
pubmed: 28334900
pmcid: 5499812
Liu M, Gou L, Xia J, Wan Q, Jiang Y, Sun S, Tang M, He T, Zhang Y (2018) LncRNA ITGB2-AS1 could promote the migration and invasion of breast cancer cells through up-regulating ITGB2. Int J Mol Sci 19(7):1866. https://doi.org/10.3390/ijms19071866
doi: 10.3390/ijms19071866
pmcid: 6073814
Wu B, Wang H, Zhang L, Sun C, Li H, Jiang C, Liu X (2019) High expression of RAD18 in glioma induces radiotherapy resistance via down-regulating P53 expression. Biomed Pharmacotherap 112:108555. https://doi.org/10.1016/j.biopha.2019.01.016
doi: 10.1016/j.biopha.2019.01.016
Zou S, Yang J, Guo J, Su Y, He C, Wu J, Yu L, Ding WQ, Zhou J (2018) RAD18 promotes the migration and invasion of esophageal squamous cell cancer via the JNK-MMPs pathway. Cancer Lett 417:65–74. https://doi.org/10.1016/j.canlet.2017.12.034
doi: 10.1016/j.canlet.2017.12.034
pubmed: 29306013
Lou P, Zou S, Shang Z, He C, Gao A, Hou S, Zhou J (2019) RAD18 contributes to the migration and invasion of human cervical cancer cells via the interleukin1beta pathway. Mol Med Reports 20(4):3415–3423. https://doi.org/10.3892/mmr.2019.10564
doi: 10.3892/mmr.2019.10564
Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA: Cancer J Clin 65(2):87–108. https://doi.org/10.3322/caac.21262
doi: 10.3322/caac.21262
Jin Y, Feng SJ, Qiu S, Shao N, Zheng JH (2017) LncRNA MALAT1 promotes proliferation and metastasis in epithelial ovarian cancer via the PI3K-AKT pathway. Eur Rev Med Pharmacol Sci 21(14):3176–3184
pubmed: 28770968
Wang J, Xu W, He Y, Xia Q, Liu S (2018) LncRNA MEG3 impacts proliferation, invasion, and migration of ovarian cancer cells through regulating PTEN. Inflamm Res 67(11–12):927–936. https://doi.org/10.1007/s00011-018-1186-z
doi: 10.1007/s00011-018-1186-z
pubmed: 30310931
Liang H, Yu T, Han Y, Jiang H, Wang C, You T, Zhao X, Shan H, Yang R, Yang L, Shan H, Gu Y (2018) LncRNA PTAR promotes EMT and invasion-metastasis in serous ovarian cancer by competitively binding miR-101-3p to regulate ZEB1 expression. Molecular Cancer 17(1):119. https://doi.org/10.1186/s12943-018-0870-5
doi: 10.1186/s12943-018-0870-5
pubmed: 30098599
pmcid: 6087007
Xue M, Shi D, Xu G, Wang W (2019) The long noncoding RNA linc00858 promotes progress of lung cancer through miR-3182/MMP2 axis. Artif Cells Nanomed Biotechnol 47(1):2091–2097. https://doi.org/10.1080/21691401.2019.1617728
doi: 10.1080/21691401.2019.1617728
pubmed: 31131637
Xu T, Wu K, Zhang L, Zheng S, Wang X, Zuo H, Wu X, Tao G, Jiang B, Zhang L (2019) Long non-coding RNA LINC00858 exerts a tumor-promoting role in colon cancer via HNF4alpha and WNK2 regulation. Cell Oncol (Dordrecht). https://doi.org/10.1007/s13402-019-00490-8
doi: 10.1007/s13402-019-00490-8
Chi J, Liu T, Shi C, Luo H, Wu Z, Xiong B, Liu S, Zeng Y (2019) Long non-coding RNA LUCAT1 promotes proliferation and invasion in gastric cancer by regulating miR-134–5p/YWHAZ axis. Biomed Pharmacother. 118:109201. https://doi.org/10.1016/j.biopha.2019.109201
doi: 10.1016/j.biopha.2019.109201
pubmed: 31545227
Tong H, Zhao K, Wang J, Xu H, Xiao J (2020) CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell. J Pharm Pharmacol 72(1):68–75. https://doi.org/10.1111/jphp.13188
doi: 10.1111/jphp.13188
pubmed: 31721211
Xie C, Lu D, Xu M, Qu Z, Zhang W, Wang H (2019) Knockdown of RAD18 inhibits glioblastoma development. J Cell Physiol 234(11):21100–21112. https://doi.org/10.1002/jcp.28713
doi: 10.1002/jcp.28713
pubmed: 31081138