Substrate-Dependent Mobile Loop Conformational Changes in Alkanesulfonate Monooxygenase from Accelerated Molecular Dynamics.


Journal

Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623

Informations de publication

Date de publication:
29 09 2020
Historique:
pubmed: 4 9 2020
medline: 31 3 2021
entrez: 4 9 2020
Statut: ppublish

Résumé

Substrate-induced conformational changes present in alkanesulfonate monooxygenase (SsuD) are crucial to catalysis and lead to distinct interactions between a dynamic loop region and the active site. Accelerated molecular dynamics (aMD) simulations have been carried out to examine this potential correlation by studying wild-type SsuD and variant enzymes bound with different combinations of reduced flavin (FMNH

Identifiants

pubmed: 32881481
doi: 10.1021/acs.biochem.0c00633
doi:

Substances chimiques

Alkanesulfonic Acids 0
C4a-hydroperoxyflavin 0
Escherichia coli Proteins 0
Flavins 0
1-octanesulfonic acid DU4821I15A
Mixed Function Oxygenases EC 1.-
SsuD protein, E coli EC 1.14.14.5

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

3582-3593

Auteurs

Abhishek Thakur (A)

Department of Chemistry, University of Miami, Coral Gables, Florida 33146, United States.

Shruti Somai (S)

Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849, United States.

Kun Yue (K)

Department of Chemistry, University of Miami, Coral Gables, Florida 33146, United States.

Nicole Ippolito (N)

Department of Chemistry, University of Miami, Coral Gables, Florida 33146, United States.

Dianne Pagan (D)

Department of Chemistry, University of Miami, Coral Gables, Florida 33146, United States.

Jingyuan Xiong (J)

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.

Holly R Ellis (HR)

Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849, United States.

Orlando Acevedo (O)

Department of Chemistry, University of Miami, Coral Gables, Florida 33146, United States.

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Classifications MeSH