Adherens junction regulates cryptic lamellipodia formation for epithelial cell migration.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
05 10 2020
05 10 2020
Historique:
received:
30
06
2020
revised:
22
07
2020
accepted:
27
07
2020
entrez:
5
9
2020
pubmed:
5
9
2020
medline:
25
3
2021
Statut:
ppublish
Résumé
Collective migration of epithelial cells plays crucial roles in various biological processes such as cancer invasion. In migrating epithelial sheets, leader cells form lamellipodia to advance, and follower cells also form similar motile apparatus at cell-cell boundaries, which are called cryptic lamellipodia (c-lamellipodia). Using adenocarcinoma-derived epithelial cells, we investigated how c-lamellipodia form and found that they sporadically grew from around E-cadherin-based adherens junctions (AJs). WAVE and Arp2/3 complexes were localized along the AJs, and silencing them not only interfered with c-lamellipodia formation but also prevented follower cells from trailing the leaders. Disruption of AJs by removing αE-catenin resulted in uncontrolled c-lamellipodia growth, and this was brought about by myosin II activation and the resultant contraction of AJ-associated actomyosin cables. Additional observations indicated that c-lamellipodia tended to grow at mechanically weak sites of the junction. We conclude that AJs not only tie cells together but also support c-lamellipodia formation by recruiting actin regulators, enabling epithelial cells to undergo ordered collective migration.
Identifiants
pubmed: 32886101
pii: 152072
doi: 10.1083/jcb.202006196
pmc: PMC7659716
pii:
doi:
Substances chimiques
Actin-Related Protein 2-3 Complex
0
Actins
0
Cadherins
0
WASF1 protein, human
0
Wiskott-Aldrich Syndrome Protein Family
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Ozawa et al.
Références
J Biol Chem. 2007 Dec 7;282(49):35666-78
pubmed: 17932039
Science. 2019 Aug 16;365(6454):705-710
pubmed: 31416964
Dev Cell. 2019 Oct 21;51(2):158-172.e4
pubmed: 31639367
Curr Opin Cell Biol. 2004 Apr;16(2):140-5
pubmed: 15196556
Breast Cancer Res Treat. 2012 Apr;132(2):641-52
pubmed: 22080244
J Cell Biol. 2012 Oct 29;199(3):545-63
pubmed: 23091067
Mol Biol Cell. 2012 Dec;23(23):4601-10
pubmed: 23051739
Cell. 2018 Aug 9;174(4):856-869.e17
pubmed: 30096312
Nat Rev Mol Cell Biol. 2014 Sep;15(9):577-90
pubmed: 25145849
Cancer Cell. 2006 Nov;10(5):437-49
pubmed: 17097565
Cell. 2014 May 22;157(5):1146-59
pubmed: 24855950
J Cell Biol. 2020 May 4;219(5):
pubmed: 32232465
Dev Cell. 2019 Feb 25;48(4):445-459.e5
pubmed: 30773490
Nat Mater. 2017 May;16(5):587-596
pubmed: 28135264
Nat Commun. 2013;4:2531
pubmed: 24100690
Elife. 2017 Sep 26;6:
pubmed: 28949297
J Cell Biol. 2006 Oct 9;175(1):135-46
pubmed: 17015620
J Cell Biol. 1998 Aug 10;142(3):847-57
pubmed: 9700171
J Cell Biol. 1994 Oct;127(1):247-56
pubmed: 7929567
Nat Mater. 2019 Nov;18(11):1252-1263
pubmed: 31332337
Mol Biol Cell. 2004 Aug;15(8):3509-19
pubmed: 15169870
Nat Cell Biol. 2015 Mar;17(3):276-87
pubmed: 25706233
J Cell Biol. 2016 Feb 29;212(5):591-603
pubmed: 26903538
J Cell Biol. 2016 Nov 21;215(4):559-573
pubmed: 27807130
Science. 2014 Oct 24;346(6208):1257998
pubmed: 25342811
Curr Biol. 2002 Mar 5;12(5):379-82
pubmed: 11882288
Dev Cell. 2014 Sep 29;30(6):673-87
pubmed: 25199687
Science. 2003 Mar 14;299(5613):1743-7
pubmed: 12637748
Nat Rev Mol Cell Biol. 2014 Jun;15(6):397-410
pubmed: 24824068
Trends Cell Biol. 2015 Sep;25(9):556-66
pubmed: 26137890
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10788-93
pubmed: 12960404
Nat Rev Mol Cell Biol. 2007 Jan;8(1):37-48
pubmed: 17183359
Dev Cell. 2010 Jul 20;19(1):39-53
pubmed: 20643349
Nat Rev Mol Cell Biol. 2009 Jul;10(7):445-57
pubmed: 19546857
Nat Rev Mol Cell Biol. 2013 Jan;14(1):7-12
pubmed: 23212475
Nat Rev Mol Cell Biol. 2016 Feb;17(2):97-109
pubmed: 26726037
Mol Biol Cell. 2005 Feb;16(2):701-16
pubmed: 15548593
Nat Rev Mol Cell Biol. 2017 Dec;18(12):743-757
pubmed: 29115298
J Cell Sci. 2005 Jan 1;118(Pt 1):51-63
pubmed: 15585576
Nat Cell Biol. 2012 Aug;14(8):777-83
pubmed: 22854810
Nat Cell Biol. 2007 Jan;9(1):92-8
pubmed: 17159998
Biol Open. 2018 Apr 30;7(5):
pubmed: 29654115
Nat Rev Mol Cell Biol. 2009 Nov;10(11):778-90
pubmed: 19851336
Mol Biol Cell. 2018 Feb 15;29(4):380-388
pubmed: 29282282
Curr Opin Cell Biol. 2003 Dec;15(6):740-6
pubmed: 14644200
J Cell Biol. 1999 Feb 8;144(3):533-47
pubmed: 9971747
Mol Biol Cell. 2012 Oct;23(20):4020-31
pubmed: 22918944
J Cell Sci. 2009 Apr 1;122(Pt 7):905-11
pubmed: 19258396
Cell Mol Life Sci. 2016 Mar;73(6):1119-30
pubmed: 26585026
Nat Cell Biol. 2020 Jan;22(1):97-107
pubmed: 31907411
Mol Biol Cell. 2007 Feb;18(2):605-16
pubmed: 17151359
Cold Spring Harb Perspect Biol. 2017 May 1;9(5):
pubmed: 28096263
Nature. 2019 Sep;573(7774):439-444
pubmed: 31485072
J Cell Sci. 2012 Feb 15;125(Pt 4):844-57
pubmed: 22275437
Biochem Biophys Res Commun. 2015 Mar 13;458(3):608-613
pubmed: 25681770
J Biol Chem. 2014 Mar 14;289(11):7764-75
pubmed: 24469447
Mol Biol Cell. 2017 Jul 15;28(15):2023-2034
pubmed: 28705832
Dev Cell. 2009 Nov;17(5):736-43
pubmed: 19879198
Mol Oncol. 2017 Jan;11(1):5-27
pubmed: 28085224
Curr Opin Cell Biol. 2014 Oct;30:99-111
pubmed: 25137487
Nat Genet. 2014 Jun;46(6):573-82
pubmed: 24816253
J Biol Chem. 2004 Aug 6;279(32):34062-70
pubmed: 15159390
J Cell Biol. 1963 May;17:375-412
pubmed: 13944428
Nature. 1984 Jul 5-11;310(5972):58-61
pubmed: 6377086
J Cell Sci. 2007 Jan 1;120(Pt 1):86-100
pubmed: 17164293
Dev Cell. 2017 Mar 13;40(5):467-477.e5
pubmed: 28292425
Curr Opin Cell Biol. 2015 Oct;36:103-12
pubmed: 26363959
Nat Methods. 2008 Jul;5(7):605-7
pubmed: 18536722
J Cell Biol. 2009 Jun 1;185(5):779-86
pubmed: 19487453
Mol Biol Cell. 2017 Jul 7;28(14):1833-1846
pubmed: 28684609