Cross-utilisation of template RNAs by alphavirus replicases.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
28
02
2020
accepted:
21
07
2020
revised:
17
09
2020
pubmed:
5
9
2020
medline:
21
10
2020
entrez:
4
9
2020
Statut:
epublish
Résumé
Most alphaviruses (family Togaviridae) including Sindbis virus (SINV) and other human pathogens, are transmitted by arthropods. The first open reading frame in their positive strand RNA genome encodes for the non-structural polyprotein, a precursor to four separate subunits of the replicase. The replicase interacts with cis-acting elements located near the intergenic region and at the ends of the viral RNA genome. A trans-replication assay was developed and used to analyse the template requirements for nine alphavirus replicases. Replicases of alphaviruses of the Semliki Forest virus complex were able to cross-utilize each other's templates as well as those of outgroup alphaviruses. Templates of outgroup alphaviruses, including SINV and the mosquito-specific Eilat virus, were promiscuous; in contrast, their replicases displayed a limited capacity to use heterologous templates, especially in mosquito cells. The determinants important for efficient replication of template RNA were mapped to the 5' region of the genome. For SINV these include the extreme 5'- end of the genome and sequences corresponding to the first stem-loop structure in the 5' untranslated region. Mutations introduced in these elements drastically reduced infectivity of recombinant SINV genomes. The trans-replicase tools and approaches developed here can be instrumental in studying alphavirus recombination and evolution, but can also be applied to study other viruses such as picornaviruses, flaviviruses and coronaviruses.
Identifiants
pubmed: 32886709
doi: 10.1371/journal.ppat.1008825
pii: PPATHOGENS-D-20-00382
pmc: PMC7498090
doi:
Substances chimiques
RNA, Viral
0
Viral Proteins
0
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008825Subventions
Organisme : Wellcome Trust
ID : 200171/Z/15/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007033
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007038
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007039
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/NO1054X/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Virol. 1993 Nov;67(11):6439-46
pubmed: 8411346
RNA. 2001 Nov;7(11):1638-51
pubmed: 11720292
Proc Natl Acad Sci U S A. 2019 May 7;116(19):9558-9567
pubmed: 31000599
J Virol. 2005 Apr;79(8):4630-9
pubmed: 15795249
Cell. 1986 Jan 17;44(1):137-45
pubmed: 3753584
Virology. 1994 Mar;199(2):366-75
pubmed: 7510084
J Virol. 2020 Mar 17;94(7):
pubmed: 31941782
J Virol. 1992 Feb;66(2):857-64
pubmed: 1309918
EMBO J. 1994 Jun 15;13(12):2925-34
pubmed: 7517863
Microbiol Rev. 1994 Sep;58(3):491-562
pubmed: 7968923
J Mol Biol. 1983 Jul 25;168(1):1-15
pubmed: 6308269
J Virol. 2018 Jun 29;92(14):
pubmed: 29695431
Virus Res. 2015 Aug 3;206:99-107
pubmed: 25630058
J Gen Virol. 2008 Mar;89(Pt 3):676-686
pubmed: 18272758
Parasit Vectors. 2015 Sep 17;8:464
pubmed: 26384002
Annu Rev Entomol. 2002;47:1-31
pubmed: 11729067
J Virol. 2015 Mar;89(6):3145-62
pubmed: 25552719
PLoS Pathog. 2019 Jan 22;15(1):e1007239
pubmed: 30668592
J Virol. 2006 Jun;80(12):5686-96
pubmed: 16731907
PLoS Pathog. 2013;9(9):e1003610
pubmed: 24039580
Lancet Infect Dis. 2017 Apr;17(4):e107-e117
pubmed: 28159534
J Virol. 2014 May;88(10):5888-93
pubmed: 24623412
Virus Evol. 2018 Jun 04;4(1):vey012
pubmed: 29942653
Vector Borne Zoonotic Dis. 2010 Nov;10(9):889-907
pubmed: 20420530
Am J Trop Med Hyg. 2016 Sep 7;95(3):633-8
pubmed: 27402516
Trans R Soc Trop Med Hyg. 2012 Dec;106(12):749-55
pubmed: 23122869
PLoS One. 2009;4(3):e4772
pubmed: 19274090
mBio. 2018 Aug 21;9(4):
pubmed: 30131356
J Med Entomol. 2019 Apr 16;56(3):832-843
pubmed: 30668762
J Virol. 2012 Jan;86(1):553-65
pubmed: 22031949
Virology. 1993 Nov;197(1):375-90
pubmed: 8105605
PLoS Pathog. 2016 Aug 10;12(8):e1005810
pubmed: 27509095
J Virol. 2011 May;85(10):4739-51
pubmed: 21389137
Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14622-7
pubmed: 22908261
PLoS One. 2016 Mar 10;11(3):e0151616
pubmed: 26963103
PLoS Negl Trop Dis. 2017 Aug 3;11(8):e0005693
pubmed: 28771475
Vaccine. 2012 Jun 29;30(31):4638-43
pubmed: 22583812
Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):E10457-E10466
pubmed: 30322911
Nucleic Acids Res. 2019 Sep 26;47(17):9296-9312
pubmed: 31350895
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
pubmed: 12824337
Science. 2014 Feb 14;343(6172):783-7
pubmed: 24482115
J Virol. 2019 Aug 28;93(18):
pubmed: 31217251
Pathog Glob Health. 2017 Sep;111(6):271-275
pubmed: 28829253
PLoS Negl Trop Dis. 2018 Nov 7;12(11):e0006895
pubmed: 30403665
Nature. 2019 Oct;574(7777):259-263
pubmed: 31554973
PLoS Negl Trop Dis. 2013;7(1):e1931
pubmed: 23359824
J Gen Virol. 2018 Jun;99(6):761-762
pubmed: 29745869
Euro Surveill. 2010 May 06;15(18):
pubmed: 20460093
J Virol. 2007 Apr;81(8):4371-3
pubmed: 17287268
Biotechnology (N Y). 1991 Dec;9(12):1356-61
pubmed: 1370252
J Virol. 2007 Jul;81(13):7061-8
pubmed: 17442713
Antiviral Res. 2017 Jul;143:38-47
pubmed: 28390873
J Gen Virol. 2015 Sep;96(9):2483-2500
pubmed: 26219641
PLoS Negl Trop Dis. 2015 Feb 06;9(2):e0003436
pubmed: 25658762
J Virol. 1990 Sep;64(9):4162-8
pubmed: 2384916
J Virol. 2010 Aug;84(15):7543-57
pubmed: 20484502
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5235-9
pubmed: 6291034
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9429-34
pubmed: 15197279
Cell Host Microbe. 2010 Aug 19;8(2):196-207
pubmed: 20709296
J Virol. 2003 Feb;77(4):2301-9
pubmed: 12551967
Virology. 2015 Jan 1;474:154-62
pubmed: 25463613
J Virol. 2004 May;78(10):4953-64
pubmed: 15113874
J Virol. 2007 Mar;81(5):2472-84
pubmed: 17108023
Bioinformatics. 2009 Aug 1;25(15):1974-5
pubmed: 19398448
J Virol. 2018 Jun 29;92(14):
pubmed: 29695432
J Virol. 2020 Mar 31;94(8):
pubmed: 31996431
J Virol. 2004 Jan;78(1):61-75
pubmed: 14671088
PLoS Biol. 2019 Jan 22;17(1):e3000130
pubmed: 30668574
PLoS Pathog. 2013;9(8):e1003591
pubmed: 24009512
PLoS Negl Trop Dis. 2016 Jan 25;10(1):e0004402
pubmed: 26807575
J Virol. 1985 Apr;54(1):38-44
pubmed: 3973983
Lancet. 1996 Aug 17;348(9025):436-40
pubmed: 8709783
Front Microbiol. 2019 Sep 24;10:2236
pubmed: 31636613
J Virol. 2010 Nov;84(22):11679-95
pubmed: 20826696
J Virol. 2018 Nov 12;92(23):
pubmed: 30232189