The Phage-Encoded
host transcriptional shutdown
phage-induced acetylation
phage–host interactions
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
02 09 2020
02 09 2020
Historique:
received:
06
07
2020
revised:
26
08
2020
accepted:
01
09
2020
entrez:
5
9
2020
pubmed:
6
9
2020
medline:
10
3
2021
Statut:
epublish
Résumé
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses. These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. Rac mediates the inactivation of bacterial transcription by introducing a specific cleavage in the α subunit of the bacterial RNA polymerase. This cleavage occurs within the flexible linker sequence and disconnects the C-terminal domain, required for transcription initiation from most highly active cellular promoters. To achieve this, Rac likely taps into a novel post-translational modification (PTM) mechanism within the host
Identifiants
pubmed: 32887488
pii: v12090976
doi: 10.3390/v12090976
pmc: PMC7552054
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Viral Proteins
0
Acetyltransferases
EC 2.3.1.-
DNA-Directed RNA Polymerases
EC 2.7.7.6
RNA polymerase alpha subunit
EC 2.7.7.6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCRR NIH HHS
ID : S10 RR021056
Pays : United States
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