Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA.
Betacoronavirus
/ genetics
COVID-19
COVID-19 Testing
Clinical Laboratory Techniques
/ methods
Coronavirus Infections
/ diagnosis
Diagnostic Tests, Routine
Endopeptidase K
/ chemistry
Heating
Humans
Pandemics
Pneumonia, Viral
/ diagnosis
RNA, Viral
/ genetics
Reagent Kits, Diagnostic
SARS-CoV-2
Sensitivity and Specificity
COVID-19
Heating
Kit-free RNA extraction
Proteinase K
SARS-CoV-2
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
13
05
2020
revised:
28
08
2020
accepted:
30
08
2020
pubmed:
7
9
2020
medline:
1
12
2020
entrez:
6
9
2020
Statut:
ppublish
Résumé
Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment.
Identifiants
pubmed: 32891677
pii: S0166-0934(20)30217-2
doi: 10.1016/j.jviromet.2020.113965
pmc: PMC7468345
pii:
doi:
Substances chimiques
RNA, Viral
0
Reagent Kits, Diagnostic
0
Endopeptidase K
EC 3.4.21.64
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113965Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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