Identification of a protective epitope in Japanese encephalitis virus NS1 protein.
Animals
Antibodies, Monoclonal
/ immunology
Antibodies, Viral
/ immunology
Binding Sites, Antibody
Cell Line
Cricetinae
Encephalitis Virus, Japanese
/ immunology
Encephalitis, Japanese
/ immunology
Epitope Mapping
Epitopes
/ immunology
Immunization
Kidney
/ cytology
Mice
Mice, Inbred BALB C
Peptides
/ administration & dosage
Sf9 Cells
Spodoptera
Viral Nonstructural Proteins
/ immunology
Japanese encephalitis virus (JEV)
Monoclonal antibody
Non-structural protein 1
Protective epitope
Journal
Antiviral research
ISSN: 1872-9096
Titre abrégé: Antiviral Res
Pays: Netherlands
ID NLM: 8109699
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
21
03
2020
revised:
23
08
2020
accepted:
02
09
2020
pubmed:
9
9
2020
medline:
13
7
2021
entrez:
8
9
2020
Statut:
ppublish
Résumé
Japanese encephalitis virus (JEV) is one of the most important culex transmitted-flaviviruses, which can cause encephalitis in humans. Although non-structural protein 1 (NS1) of JEV does not stimulate neutralizing antibodies, this protein can provide high immunoprotection in vivo. The protective epitopes and the protective mechanism of NS1 still remain unclear. In this study, we generated five different monoclonal antibodies (mAbs) targeting the NS1 protein of JEV. In vitro experiments revealed that none of these five antibodies neutralized the JEV infection. In mouse protection studies, one of these mAbs, designated 2B8, provided a therapeutic effect against JEV lethal challenge (70% survival rate). Using peptide mapping analysis, we found that mAb 2B8 reacted with the epitope
Identifiants
pubmed: 32898585
pii: S0166-3542(20)30344-2
doi: 10.1016/j.antiviral.2020.104930
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Epitopes
0
NS1 protein, Flavivirus
0
Peptides
0
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104930Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.