Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro-Stem Cell of Fallopian Epithelium.
Animals
Cell Culture Techniques
/ methods
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cell Separation
Cryopreservation
Culture Media
Epithelial Cells
/ cytology
Epithelium
/ growth & development
Fallopian Tubes
/ growth & development
Female
Humans
In Vitro Techniques
Intercellular Signaling Peptides and Proteins
Primary Cell Culture
Stem Cells
/ cytology
Swine
air–liquid interface (ALI)
basal stem cell
cilia
ciliary motility
differentiation
high-grade serous ovarian carcinoma (HGSC)
oviduct
pig
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
03 09 2020
03 09 2020
Historique:
received:
30
06
2020
revised:
30
08
2020
accepted:
31
08
2020
entrez:
9
9
2020
pubmed:
10
9
2020
medline:
25
6
2021
Statut:
epublish
Résumé
The fallopian tube (FT) is an important reproductive organ in females. The luminal epithelium of the FT is composed of highly polarized secretory and ciliated cells. Recently, accumulating lines of evidence have suggested that the origin of high-grade serous ovarian carcinoma (HGSC) is fallopian tube epithelial cells (FTECs). Due to the lack of a high-fidelity model for FTECs in vitro, homeostasis, differentiation, as well as the transformation of FTECs are still enigmatic. In this study, we optimized the culture condition for the stable expansion of basal stem cells, as well as inducing differentiation of basal cells into polarized secretory and ciliated cells in the air-liquid interface (ALI) condition suitable for long-term culture. This storable culture method of FTECs provides a versatile platform for studying differentiation mechanisms, intercellular communication, and transformation to HGSC, as well as the physiological function of the FT in vitro.
Identifiants
pubmed: 32899226
pii: biom10091270
doi: 10.3390/biom10091270
pmc: PMC7565394
pii:
doi:
Substances chimiques
Culture Media
0
Intercellular Signaling Peptides and Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japanese Society for the Promotion of Science
ID : 17K15543
Pays : International
Organisme : Japanese Society for the Promotion of Science
ID : 17K08511
Pays : International
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