Extracellular Matrix Injury of Kidney Allografts in Antibody-Mediated Rejection: A Proteomics Study.
Adult
Aged
Allografts
/ metabolism
Antibodies
/ metabolism
Basement Membrane
/ metabolism
Biopsy
Cathepsins
/ metabolism
Cell Line
Cysteine Endopeptidases
/ metabolism
Extracellular Matrix
/ metabolism
Female
Galectin 1
/ genetics
Gene Expression
Glutathione Transferase
/ metabolism
Graft Rejection
/ genetics
Histocompatibility Antigens Class I
/ immunology
Humans
Kidney Glomerulus
/ metabolism
Kidney Transplantation
Kidney Tubules
/ metabolism
Laminin
/ metabolism
Male
Matrix Metalloproteinase 2
/ metabolism
Matrix Metalloproteinase 3
/ metabolism
Membrane Proteins
/ metabolism
Middle Aged
Necrosis
Proteomics
Receptor-Like Protein Tyrosine Phosphatases, Class 3
/ metabolism
Tumor Necrosis Factor-alpha
/ pharmacology
acute allograft rejection
extracellular matrix
glomerular endothelial cells
kidney transplantation
proximal tubule
renal biopsy
Journal
Journal of the American Society of Nephrology : JASN
ISSN: 1533-3450
Titre abrégé: J Am Soc Nephrol
Pays: United States
ID NLM: 9013836
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
10
03
2020
accepted:
21
07
2020
pubmed:
10
9
2020
medline:
10
3
2021
entrez:
9
9
2020
Statut:
ppublish
Résumé
Antibody-mediated rejection (AMR) accounts for >50% of kidney allograft loss. Donor-specific antibodies (DSA) against HLA and non-HLA antigens in the glomeruli and the tubulointerstitium cause AMR while inflammatory cytokines such as TNF Unbiased proteomic analysis of laser-captured and microdissected glomeruli and tubulointerstitium was performed on 30 for-cause kidney biopsy specimens with early AMR, acute cellular rejection (ACR), or acute tubular necrosis (ATN). A total of 107 of 2026 glomerular and 112 of 2399 tubulointerstitial proteins was significantly differentially expressed in AMR versus ACR; 112 of 2026 glomerular and 181 of 2399 tubulointerstitial proteins were significantly dysregulated in AMR versus ATN ( Basement membranes are often remodeled in chronic AMR. Proteomic analysis performed on laser-captured and microdissected glomeruli and tubulointerstitium identified early ECM remodeling, which may represent a new therapeutic opportunity.
Sections du résumé
BACKGROUND
Antibody-mediated rejection (AMR) accounts for >50% of kidney allograft loss. Donor-specific antibodies (DSA) against HLA and non-HLA antigens in the glomeruli and the tubulointerstitium cause AMR while inflammatory cytokines such as TNF
METHODS
Unbiased proteomic analysis of laser-captured and microdissected glomeruli and tubulointerstitium was performed on 30 for-cause kidney biopsy specimens with early AMR, acute cellular rejection (ACR), or acute tubular necrosis (ATN).
RESULTS
A total of 107 of 2026 glomerular and 112 of 2399 tubulointerstitial proteins was significantly differentially expressed in AMR versus ACR; 112 of 2026 glomerular and 181 of 2399 tubulointerstitial proteins were significantly dysregulated in AMR versus ATN (
CONCLUSIONS
Basement membranes are often remodeled in chronic AMR. Proteomic analysis performed on laser-captured and microdissected glomeruli and tubulointerstitium identified early ECM remodeling, which may represent a new therapeutic opportunity.
Identifiants
pubmed: 32900843
pii: ASN.2020030286
doi: 10.1681/ASN.2020030286
pmc: PMC7608967
doi:
Substances chimiques
Antibodies
0
Galectin 1
0
Histocompatibility Antigens Class I
0
LGALS1 protein, human
0
Laminin
0
Membrane Proteins
0
Tumor Necrosis Factor-alpha
0
laminin gamma 1
0
nephrin
0
GSTO1 protein, human
EC 2.5.1.18
Glutathione Transferase
EC 2.5.1.18
PTPRO protein, human
EC 3.1.3.48
Receptor-Like Protein Tyrosine Phosphatases, Class 3
EC 3.1.3.48
Cathepsins
EC 3.4.-
Cysteine Endopeptidases
EC 3.4.22.-
CTSV protein, human
EC 3.4.22.43
MMP3 protein, human
EC 3.4.24.17
Matrix Metalloproteinase 3
EC 3.4.24.17
MMP2 protein, human
EC 3.4.24.24
Matrix Metalloproteinase 2
EC 3.4.24.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2705-2724Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
Copyright © 2020 by the American Society of Nephrology.
Références
Mediators Inflamm. 2018 Nov 5;2018:8696543
pubmed: 30524200
J Exp Med. 1986 May 1;163(5):1064-84
pubmed: 2939168
J Am Soc Nephrol. 2019 Oct;30(10):1886-1897
pubmed: 31308073
Am J Transplant. 2007 Dec;7(12):2712-22
pubmed: 17941957
Sci Rep. 2016 Oct 11;6:35068
pubmed: 27725732
Nucleic Acids Res. 2020 Jan 8;48(D1):D479-D488
pubmed: 31733064
Immunity. 1996 Apr;4(4):357-66
pubmed: 8612130
Cardiovasc Diabetol. 2017 Apr 26;16(1):55
pubmed: 28446168
Biochem Biophys Res Commun. 2008 May 2;369(2):781-7
pubmed: 18312854
PLoS One. 2017 Sep 13;12(9):e0184781
pubmed: 28902875
PLoS One. 2015 Jul 31;10(7):e0134688
pubmed: 26230727
Kidney Int Rep. 2016 Nov 19;2(3):461-469
pubmed: 29142973
J Immunol. 2002 May 1;168(9):4488-94
pubmed: 11970993
Matrix Biol. 2017 Jan;57-58:12-28
pubmed: 27553508
J Immunol. 2005 Jun 1;174(11):7022-32
pubmed: 15905545
PLoS Pathog. 2010 Jul 15;6(7):e1000993
pubmed: 20657665
Am J Transplant. 2018 Feb;18(2):293-307
pubmed: 29243394
J Histochem Cytochem. 2019 Sep;67(9):643-661
pubmed: 31116062
Nephrol Dial Transplant. 2011 May;26(5):1483-92
pubmed: 21045077
Am J Transplant. 2010 Aug;10(8):1812-22
pubmed: 20659089
Nucleic Acids Res. 2013 Jan;41(Database issue):D1063-9
pubmed: 23203882
Am J Transplant. 2009 Oct;9(10):2312-23
pubmed: 19681822
Biochem Biophys Res Commun. 2004 Oct 8;323(1):213-7
pubmed: 15351723
Annu Rev Physiol. 1997;59:63-88
pubmed: 9074757
Br J Pharmacol. 2018 Apr;175(8):1157-1172
pubmed: 28631302
Am J Transplant. 2012 Feb;12(2):388-99
pubmed: 22081892
Curr Opin Organ Transplant. 2014 Feb;19(1):33-40
pubmed: 24316758
Antioxid Redox Signal. 2011 Jul 1;15(1):233-70
pubmed: 21235352
Transplant Proc. 2013 Nov;45(9):3262-8
pubmed: 24182797
Transplantation. 2019 Jul;103(7):1468-1476
pubmed: 30335696
J Thromb Haemost. 2017 May;15(5):1020-1031
pubmed: 28239987
J Am Soc Nephrol. 2004 Jun;15(6):1382-91
pubmed: 15153549
Fibrogenesis Tissue Repair. 2014 Mar 28;7(1):4
pubmed: 24678881
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
J Signal Transduct. 2011;2011:317852
pubmed: 21776384
Immunity. 2014 Dec 18;41(6):960-72
pubmed: 25526309
Am J Transplant. 2019 Oct;19(10):2692-2704
pubmed: 31062482
Curr Opin Organ Transplant. 2017 Apr;22(2):97-104
pubmed: 28060025
Ann Rheum Dis. 2005 Nov;64 Suppl 4:iv96-103
pubmed: 16239398
Curr Transplant Rep. 2015 Sep;2(3):222-232
pubmed: 28344919
Biomed Pharmacother. 2003 May-Jun;57(3-4):145-55
pubmed: 12818476
Lab Invest. 2006 Jun;86(6):578-90
pubmed: 16607379
J Immunol. 2007 Jun 15;178(12):7911-22
pubmed: 17548629
Haematologica. 2018 Dec;103(12):e610-e612
pubmed: 30262557
Am J Transplant. 2008 Dec;8(12):2627-35
pubmed: 19032226
Glycobiology. 2006 Nov;16(11):137R-157R
pubmed: 16840800
Kidney Int Rep. 2019 Apr 15;4(7):977-986
pubmed: 31317119
Front Endocrinol (Lausanne). 2014 Oct 14;5:160
pubmed: 25352829
Nat Rev Mol Cell Biol. 2014 Dec;15(12):786-801
pubmed: 25415508
J Immunol Res. 2017;2017:8746303
pubmed: 28255564
J Immunol. 2008 Feb 15;180(4):2357-66
pubmed: 18250445
Mol Cell Proteomics. 2017 Mar;16(3):368-385
pubmed: 28062795
J Transl Med. 2019 Jul 3;17(1):216
pubmed: 31269957
Sci Rep. 2018 Feb 9;8(1):2725
pubmed: 29426942
PLoS One. 2011;6(7):e21800
pubmed: 21789182
J Neuropathol Exp Neurol. 2001 Sep;60(9):847-55
pubmed: 11556541
Nucleic Acids Res. 2020 Jan 8;48(D1):D498-D503
pubmed: 31691815
Tissue Cell. 2017 Oct;49(5):537-544
pubmed: 28732559
Am J Transplant. 2005 Feb;5(2):383-93
pubmed: 15643999
Curr Top Membr. 2015;76:231-53
pubmed: 26610916
N Engl J Med. 2019 May 16;380(20):1918-1928
pubmed: 31091373
J Proteomics. 2017 Aug 8;165:113-118
pubmed: 28647518
Cell. 2014 Mar 13;156(6):1235-1246
pubmed: 24630725
Bioinformatics. 2009 Dec 15;25(24):3327-9
pubmed: 19837718
J Pathol Clin Res. 2019 Jul;5(3):145-153
pubmed: 30740936
Breast Cancer Res. 2011;13(6):R115
pubmed: 22093547
Biochim Biophys Acta. 2014 Aug;1840(8):2560-70
pubmed: 24680817
Nucleic Acids Res. 2019 Jan 8;47(D1):D581-D589
pubmed: 30407591
J Am Soc Nephrol. 2015 Jun;26(6):1450-65
pubmed: 25388223
Nat Immunol. 2014 Apr;15(4):323-32
pubmed: 24562310
Matrix Biol. 2001 Dec;20(8):543-53
pubmed: 11731271
Kidney Int. 1992 Jan;41(1):115-23
pubmed: 1593846
Sci Rep. 2017 Jul 17;7(1):5534
pubmed: 28717194
Biochem J. 1992 Feb 15;282 ( Pt 1):273-8
pubmed: 1540143
Cell Death Dis. 2019 Mar 8;10(3):235
pubmed: 30850581
Am J Physiol Cell Physiol. 2012 Oct 15;303(8):C872-82
pubmed: 22914643
J Am Soc Nephrol. 2015 Jun;26(6):1235-7
pubmed: 25388221
J Cell Sci. 2015 May 15;128(10):1982-90
pubmed: 25908843
Front Pediatr. 2018 Jan 29;6:11
pubmed: 29435440
Healthc Manage Forum. 2014 Spring;27(1):30-6
pubmed: 25109135
J Am Soc Nephrol. 2014 May;25(5):953-66
pubmed: 24436469
Am J Transplant. 2015 May;15(5):1336-48
pubmed: 25787894
J Clin Invest. 2000 Nov;106(10):1281-90
pubmed: 11086029
J Immunol. 1999 Jul 15;163(2):1010-8
pubmed: 10395699
Am J Transplant. 2018 Sep;18(9):2314-2321
pubmed: 29790290
Clin Sci (Lond). 2016 Apr 1;130(7):525-38
pubmed: 26733721
Int Immunopharmacol. 2010 Jun;10(6):643-7
pubmed: 20298813
Front Immunol. 2018 Jan 29;9:106
pubmed: 29434607
Am J Transplant. 2013 Apr;13(4):971-983
pubmed: 23414212
Neuropathology. 2018 Oct;38(5):529-534
pubmed: 30066398
Transplantation. 2010 Oct 15;90(7):757-64
pubmed: 20838279
Clin Transplant. 2013 Nov-Dec;27(6):790-8
pubmed: 24118527
Kidney Int. 1991 Mar;39(3):382-93
pubmed: 2062031
Nucleic Acids Res. 2018 Jan 4;46(D1):D624-D632
pubmed: 29145643
PLoS One. 2015 Dec 21;10(12):e0145306
pubmed: 26690352
Lab Invest. 2008 Oct;88(10):1024-37
pubmed: 18711355
PLoS One. 2012;7(8):e43494
pubmed: 22952693
Mol Cell Endocrinol. 2010 Oct 26;328(1-2):63-9
pubmed: 20667497
Curr Opin Organ Transplant. 2013 Jun;18(3):319-26
pubmed: 23283250
Am J Transplant. 2018 May;18(5):1096-1109
pubmed: 29045076
Sci Rep. 2018 Jun 26;8(1):9690
pubmed: 29946113
Sci Rep. 2018 Jun 22;8(1):9568
pubmed: 29934646