An LC-MS/MS- and hURAT1 cell-based approach for screening of uricosuric agents.
Animals
Chromatography, Liquid
/ methods
Dogs
Drug Evaluation, Preclinical
/ methods
Humans
Limit of Detection
Linear Models
Madin Darby Canine Kidney Cells
Organic Anion Transporters
/ antagonists & inhibitors
Organic Cation Transport Proteins
/ antagonists & inhibitors
Reproducibility of Results
Tandem Mass Spectrometry
/ methods
Uric Acid
/ analysis
Uricosuric Agents
/ analysis
LC-MS/MS
Uric acid
hURAT1
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
30 Nov 2020
30 Nov 2020
Historique:
received:
09
05
2020
revised:
10
08
2020
accepted:
16
08
2020
pubmed:
10
9
2020
medline:
30
4
2021
entrez:
9
9
2020
Statut:
ppublish
Résumé
Urate anion exchanger 1 (URAT1) expressed in the proximal renal tubules is responsible for about 90% of the reabsorption of uric acid. URAT1 is identified as an important target of uricosuric drugs. Here we present an LC-MS/MS-based approach, combined with URAT1-transgenic MDCK cells, for the assessment of uric acid. Cell lysis was executed with 50 mM NaOH to release uric acid. 1,3-
Identifiants
pubmed: 32905987
pii: S1570-0232(20)30691-7
doi: 10.1016/j.jchromb.2020.122336
pii:
doi:
Substances chimiques
Organic Anion Transporters
0
Organic Cation Transport Proteins
0
SLC22A12 protein, human
0
Uricosuric Agents
0
Uric Acid
268B43MJ25
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122336Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.