A dicamba resistance-endowing IAA16 mutation leads to significant vegetative growth defects and impaired competitiveness in kochia (Bassia scoparia)

AUX/IAA16 dicamba resistance fitness cost kochia (Bassia scoparia) replacement series study; leaf and stem morphology

Journal

Pest management science
ISSN: 1526-4998
Titre abrégé: Pest Manag Sci
Pays: England
ID NLM: 100898744

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 05 05 2020
revised: 29 08 2020
accepted: 10 09 2020
pubmed: 11 9 2020
medline: 15 1 2021
entrez: 10 9 2020
Statut: ppublish

Résumé

Precise quantification of the fitness cost of synthetic auxin resistance has been impeded by lack of knowledge about the genetic basis of resistance in weeds. Recent elucidation of a resistance-endowing IAA16 mutation (G73N) in the key weed species kochia (Bassia scoparia), allows detailed characterization of the contribution of resistance alleles to weed fitness, both in the presence and absence of herbicides. Different G73N genotypes from a segregating resistant parental line (9425) were characterized for cross-resistance to dicamba, 2,4-d and fluroxypyr, and changes on stem/leaf morphology and plant architecture. Plant competitiveness and dominance of the fitness effects was quantified through measuring biomass and seed production of three F G73N confers robust resistance to dicamba but only moderate to weak resistance to 2,4-D and fluroxypyr. G73N mutant plants displayed significant vegetative growth defects: (i) they were 30-50% shorter, with a more tumbling style plant architecture, and (ii) they had thicker and more ovate (versus lanceolate and linear) leaf blades with lower photosynthesis efficiency, and 40-60% smaller stems with less-developed vascular bundle systems. F G73N is associated with significant vegetative growth defects and reduced competitiveness in synthetic auxin-resistant kochia. Management practices should target resistant kochia's high vulnerability to competition in order to effectively contain the spread of resistance.

Sections du résumé

BACKGROUND BACKGROUND
Precise quantification of the fitness cost of synthetic auxin resistance has been impeded by lack of knowledge about the genetic basis of resistance in weeds. Recent elucidation of a resistance-endowing IAA16 mutation (G73N) in the key weed species kochia (Bassia scoparia), allows detailed characterization of the contribution of resistance alleles to weed fitness, both in the presence and absence of herbicides. Different G73N genotypes from a segregating resistant parental line (9425) were characterized for cross-resistance to dicamba, 2,4-d and fluroxypyr, and changes on stem/leaf morphology and plant architecture. Plant competitiveness and dominance of the fitness effects was quantified through measuring biomass and seed production of three F
RESULTS RESULTS
G73N confers robust resistance to dicamba but only moderate to weak resistance to 2,4-D and fluroxypyr. G73N mutant plants displayed significant vegetative growth defects: (i) they were 30-50% shorter, with a more tumbling style plant architecture, and (ii) they had thicker and more ovate (versus lanceolate and linear) leaf blades with lower photosynthesis efficiency, and 40-60% smaller stems with less-developed vascular bundle systems. F
CONCLUSION CONCLUSIONS
G73N is associated with significant vegetative growth defects and reduced competitiveness in synthetic auxin-resistant kochia. Management practices should target resistant kochia's high vulnerability to competition in order to effectively contain the spread of resistance.

Identifiants

pubmed: 32909332
doi: 10.1002/ps.6080
pmc: PMC7821297
doi:

Substances chimiques

Herbicides 0
Dicamba SJG3M6RY6H

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

795-804

Informations de copyright

© 2020 Society of Chemical Industry.

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Auteurs

Chenxi Wu (C)

Bayer CropScience, Chesterfield, MO, USA.

Sherry LeClere (S)

Bayer CropScience, Chesterfield, MO, USA.

Kang Liu (K)

Bayer CropScience, Chesterfield, MO, USA.

Marta Paciorek (M)

Bayer CropScience, Chesterfield, MO, USA.

Alejandro Perez-Jones (A)

Bayer CropScience, Chesterfield, MO, USA.

Phil Westra (P)

Department of Agricultural Biology, Colorado State University, Wentzville, MO, USA.

R Douglas Sammons (RD)

Sammons BFC LLC, Wentzville, Wentzville, MO, 63365, USA.

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Classifications MeSH