Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae.
bacterial conjugation
cryo-EM
filamentous bacteriophage
helical assemblies
mating pili
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
01 12 2020
01 12 2020
Historique:
received:
02
07
2020
revised:
17
08
2020
accepted:
21
08
2020
pubmed:
12
9
2020
medline:
5
10
2021
entrez:
11
9
2020
Statut:
ppublish
Résumé
Conjugative pili are important in mediating bacterial conjugation and horizontal gene transfer. Since plasmid transfer can include antibiotic-resistance genes, conjugation is an important mechanism in the spread of antibiotic resistance. Filamentous bacteriophages have been shown to exist in two different structural classes: those with a 5-fold rotational symmetry and those with a one-start helix with approximately 5 subunits per turn. Structures for the F and the F-like pED208 conjugation pilus have shown that they have 5-fold rotational symmetry. Here, we report the cryoelectron-microscopic structure of conjugative pili from carbapenem-resistant Klebsiella pneumoniae, encoded on the IncFIIK pKpQIL plasmid, at 3.9 Å resolution and show that it has a one-start helix. These results establish that conjugation pili can exist in at least two structural classes, consistent with other results showing that relatively small perturbations are needed to change the helical symmetry of polymers.
Identifiants
pubmed: 32916103
pii: S0969-2126(20)30292-6
doi: 10.1016/j.str.2020.08.010
pmc: PMC7710920
mid: NIHMS1625985
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1321-1328.e2Subventions
Organisme : Wellcome Trust
ID : 107057/Z/15/Z
Pays : United Kingdom
Organisme : NCRR NIH HHS
ID : G20 RR031199
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122510
Pays : United States
Organisme : Medical Research Council
ID : MR/P028225/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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