Alteration of gene expression in mice after glaucoma filtration surgery.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 09 2020
14 09 2020
Historique:
received:
04
06
2020
accepted:
24
08
2020
entrez:
15
9
2020
pubmed:
16
9
2020
medline:
15
12
2020
Statut:
epublish
Résumé
To clarify the early alterations of gene expression using a mouse model of glaucoma filtration surgery, we carried out microarray expression analysis. Using BALB/c mice, a filtration surgery model was made by incision of the limbal conjunctiva, followed by the insertion of a 33G needle tip into the anterior chamber, and 11-0 nylon sutures. Subgroups of mice were treated intraoperatively with 0.4 mg/ml mitomycin-C (MMC). At day 3 after surgery the bleb was maintained. The bleb region tissue was sampled 3 days after the filtration surgery, and gene expression analysis was carried out using a mouse Agilent 8 × 60 K array. We found 755 hyperexpressed transcripts in the bleb region compared to control conjunctiva. The hyperexpressed transcripts included epithelial cell metaplasia-related (Il1b, Krt16, Sprr1b), inflammation-related (Ccl2, Il6) and wound healing-related (Lox, Timp1) genes. We also found downregulation of a goblet cell marker gene (Gp2) in the bleb conjunctiva. MMC treatment suppressed elastin (Eln) gene expression and enhanced keratinization-related gene expression (Krt1, Lor) in the bleb region. Our results suggest the importance of epithelial wound healing after filtration surgery, and this filtration surgery model will be a useful tool for further pathophysiological analysis.
Identifiants
pubmed: 32929145
doi: 10.1038/s41598-020-72036-0
pii: 10.1038/s41598-020-72036-0
pmc: PMC7490364
doi:
Substances chimiques
Anti-Bacterial Agents
0
Mitomycin
50SG953SK6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15036Références
Weinreb, R. N., Aung, T. & Medeiros, F. A. The pathophysiology and treatment of glaucoma: a review. JAMA 311, 1901–1911. https://doi.org/10.1001/jama.2014.3192 (2014).
pubmed: 24825645
pmcid: 4523637
doi: 10.1001/jama.2014.3192
Jonas, J. B. et al. Glaucoma. Lancet 390, 2183–2193. https://doi.org/10.1016/S0140-6736(17)31469-1 (2017).
pubmed: 28577860
doi: 10.1016/S0140-6736(17)31469-1
Schlunck, G., Meyer-ter-Vehn, T., Klink, T. & Grehn, F. Conjunctival fibrosis following filtering glaucoma surgery. Exp. Eye Res. 142, 76–82. https://doi.org/10.1016/j.exer.2015.03.021 (2016).
pubmed: 26675404
doi: 10.1016/j.exer.2015.03.021
Popp, M. P. et al. Development of a microarray chip for gene expression in rabbit ocular research. Mol. Vis. 13, 164–173 (2007).
pubmed: 17293780
pmcid: 2536532
Esson, D. W., Popp, M. P., Liu, L., Schultz, G. S. & Sherwood, M. B. Microarray analysis of the failure of filtering blebs in a rat model of glaucoma filtering surgery. Invest. Ophthalmol. Vis. Sci. 45, 4450–4462. https://doi.org/10.1167/iovs.04-0375 (2004).
pubmed: 15557454
doi: 10.1167/iovs.04-0375
Picht, G., Welge-Luessen, U., Grehn, F. & Lutjen-Drecoll, E. Transforming growth factor beta 2 levels in the aqueous humor in different types of glaucoma and the relation to filtering bleb development. Graefes Arch. Clin. Exp. Ophthalmol. 239, 199–207. https://doi.org/10.1007/s004170000252 (2001).
pubmed: 11405069
doi: 10.1007/s004170000252
Lopilly Park, H. Y., Kim, J. H., Ahn, M. D. & Park, C. K. Level of vascular endothelial growth factor in tenon tissue and results of glaucoma surgery. Arch. Ophthalmol. 130, 685–689. https://doi.org/10.1001/archophthalmol.2011.2799 (2012).
pubmed: 22332204
doi: 10.1001/archophthalmol.2011.2799
Park, H. Y., Kim, J. H. & Park, C. K. Lysyl oxidase-like 2 level and glaucoma surgical outcomes. Invest. Ophthalmol. Vis. Sci. 55, 3337–3343. https://doi.org/10.1167/iovs.14-14027 (2014).
pubmed: 24764069
doi: 10.1167/iovs.14-14027
Bindlish, R. et al. Efficacy and safety of mitomycin-C in primary trabeculectomy: five-year follow-up. Ophthalmology 109, 1336–1341. https://doi.org/10.1016/s0161-6420(02)01069-2 (2002).
pubmed: 12093659
doi: 10.1016/s0161-6420(02)01069-2
Mietz, H., Chevez-Barrios, P. & Lieberman, M. W. A mouse model to study the wound healing response following filtration surgery. Graefes Arch. Clin. Exp. Ophthalmol. 236, 467–475. https://doi.org/10.1007/s004170050107 (1998).
pubmed: 9646093
doi: 10.1007/s004170050107
Seet, L. F. et al. SPARC deficiency results in improved surgical survival in a novel mouse model of glaucoma filtration surgery. PLoS ONE 5, e9415. https://doi.org/10.1371/journal.pone.0009415 (2010).
pubmed: 20195533
pmcid: 2828474
doi: 10.1371/journal.pone.0009415
Van Bergen, T. et al. Inhibition of placental growth factor improves surgical outcome of glaucoma surgery. J. Cell Mol. Med. 17, 1632–1643. https://doi.org/10.1111/jcmm.12151 (2013).
pubmed: 24118824
pmcid: 3914639
doi: 10.1111/jcmm.12151
Li, S. et al. Small proline-rich protein 1B (SPRR1B) is a biomarker for squamous metaplasia in dry eye disease. Invest. Ophthalmol. Vis. Sci. 49, 34–41. https://doi.org/10.1167/iovs.07-0685 (2008).
pubmed: 18172072
pmcid: 2574421
doi: 10.1167/iovs.07-0685
Steinert, P. M., Candi, E., Kartasova, T. & Marekov, L. Small proline-rich proteins are cross-bridging proteins in the cornified cell envelopes of stratified squamous epithelia. J. Struct. Biol. 122, 76–85. https://doi.org/10.1006/jsbi.1998.3957 (1998).
pubmed: 9724607
doi: 10.1006/jsbi.1998.3957
Chang, L., Crowston, J. G., Cordeiro, M. F., Akbar, A. N. & Khaw, P. T. The role of the immune system in conjunctival wound healing after glaucoma surgery. Surv. Ophthalmol. 45, 49–68 (2000).
pubmed: 10946081
doi: 10.1016/S0039-6257(00)00135-1
Seibold, L. K., Sherwood, M. B. & Kahook, M. Y. Wound modulation after filtration surgery. Surv. Ophthalmol. 57, 530–550. https://doi.org/10.1016/j.survophthal.2012.01.008 (2012).
pubmed: 23068975
doi: 10.1016/j.survophthal.2012.01.008
Yu-Wai-Man, C. et al. Genome-wide RNA-sequencing analysis identifies a distinct fibrosis gene signature in the conjunctiva after glaucoma surgery. Sci. Rep. 7, 5644. https://doi.org/10.1038/s41598-017-05780-5 (2017).
pubmed: 28717200
pmcid: 5514109
doi: 10.1038/s41598-017-05780-5
Julia, P., Farres, J. & Pares, X. Ocular alcohol dehydrogenase in the rat: regional distribution and kinetics of the ADH-1 isoenzyme with retinol and retinal. Exp. Eye Res. 42, 305–314. https://doi.org/10.1016/0014-4835(86)90023-0 (1986).
pubmed: 2940107
doi: 10.1016/0014-4835(86)90023-0
Modis, L. Jr., Marshall, G. E. & Lee, W. R. Distribution of antioxidant enzymes in the normal aged human conjunctiva: an immunocytochemical study. Graefes Arch. Clin. Exp. Ophthalmol. 236, 86–90. https://doi.org/10.1007/s004170050047 (1998).
pubmed: 9498118
doi: 10.1007/s004170050047
Lessard, J. C. et al. Keratin 16 regulates innate immunity in response to epidermal barrier breach. Proc. Natl. Acad. Sci. USA 110, 19537–19542. https://doi.org/10.1073/pnas.1309576110 (2013).
pubmed: 24218583
doi: 10.1073/pnas.1309576110
DeBry, P. W., Perkins, T. W., Heatley, G., Kaufman, P. & Brumback, L. C. Incidence of late-onset bleb-related complications following trabeculectomy with mitomycin. Arch. Ophthalmol. 120, 297–300. https://doi.org/10.1001/archopht.120.3.297 (2002).
doi: 10.1001/archopht.120.3.297
Shields, M. B., Scroggs, M. W., Sloop, C. M. & Simmons, R. B. Clinical and histopathologic observations concerning hypotony after trabeculectomy with adjunctive mitomycin C. Am. J. Ophthalmol. 116, 673–683. https://doi.org/10.1016/s0002-9394(14)73465-8 (1993).
pubmed: 8250068
doi: 10.1016/s0002-9394(14)73465-8
Agnifili, L. et al. In vivo goblet cell density as a potential indicator of glaucoma filtration surgery outcome. Invest. Ophthalmol. Vis. Sci. 57, 2928–2935. https://doi.org/10.1167/iovs.16-19257 (2016).
pubmed: 27249666
doi: 10.1167/iovs.16-19257
Amar, N., Labbe, A., Hamard, P., Dupas, B. & Baudouin, C. Filtering blebs and aqueous pathway an immunocytological and in vivo confocal microscopy study. Ophthalmology 115, 1154–1161. https://doi.org/10.1016/j.ophtha.2007.10.024 (2008).
pubmed: 18096232
doi: 10.1016/j.ophtha.2007.10.024
Rao, K. S., Babu, K. K. & Gupta, P. D. Keratins and skin disorders. Cell Biol. Int. 20, 261–274 (1996).
pubmed: 8664850
doi: 10.1006/cbir.1996.0029
Nakamura, T. et al. Elevated expression of transglutaminase 1 and keratinization-related proteins in conjunctiva in severe ocular surface disease. Invest. Ophthalmol. Vis. Sci. 42, 549–556 (2001).
pubmed: 11222510
Matsuda, A., Tagawa, Y. & Matsuda, H. Cytokeratin and proliferative cell nuclear antigen expression in superior limbic keratoconjunctivitis. Curr. Eye Res. 15, 1033–1038. https://doi.org/10.3109/02713689609017652 (1996).
pubmed: 8921242
doi: 10.3109/02713689609017652
Santos, M., Bravo, A., Lopez, C., Paramio, J. M. & Jorcano, J. L. Severe abnormalities in the oral mucosa induced by suprabasal expression of epidermal keratin K10 in transgenic mice. J. Biol. Chem. 277, 35371–35377. https://doi.org/10.1074/jbc.M205143200 (2002).
pubmed: 12119299
doi: 10.1074/jbc.M205143200
Noble, B. A. et al. Comparison of autologous serum eye drops with conventional therapy in a randomised controlled crossover trial for ocular surface disease. Br. J. Ophthalmol. 88, 647–652. https://doi.org/10.1136/bjo.2003.026211 (2004).
pubmed: 15090417
pmcid: 1772131
doi: 10.1136/bjo.2003.026211
Kinoshita, S., Kawasaki, S., Kitazawa, K. & Shinomiya, K. Establishment of a human conjunctival epithelial cell line lacking the functional TACSTD2 gene (an American Ophthalmological Society thesis). Trans. Am. Ophthalmol. Soc. 110, 166–177 (2012).
pubmed: 23818740
pmcid: 3671362
Iwamoto, S. et al. Interaction between conjunctival epithelial cells and mast cells induces CCL2 expression and piecemeal degranulation in mast cells. Invest. Ophthalmol. Vis. Sci. 54, 2465–2473. https://doi.org/10.1167/iovs.12-10664 (2013).
pubmed: 23482464
doi: 10.1167/iovs.12-10664
Hoover, J. L., Bond, C. E., Hoover, D. B. & Defoe, D. M. Effect of neurturin deficiency on cholinergic and catecholaminergic innervation of the murine eye. Exp. Eye Res. 122, 32–39. https://doi.org/10.1016/j.exer.2014.03.002 (2014).
pubmed: 24657391
doi: 10.1016/j.exer.2014.03.002